検索結果 47530 件
タイトル(別表記) | Sources of Resistance to Net Blotch in Barley Germplasm |
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フルテキストURL | 002_001_091_102.pdf |
著者 | 佐藤 和広| 武田 和義| |
抄録 | Net blotch caused by a fungus Pyrenophora teres Drechs. is a common disease in barley. Its source of resistance has been screened by many researchers by field evaluations or seedling tests inoculating a single isolatc. Since the pathogcnic variation of isolates has been reported in net blotch, resistance of the varieties to the disease may be different among the isolates with different pathogenicities. In this study, the pathogenic variation was examined and the varietal variation of the resistance was evaluated by inoculating with four P. teres isolates collected from Japan and Canada to more than 2,200 barley varieties of the world collection preserved at the Barley Germplasm Center of Okayama University. A preliminary inoculation test showed that the disease rating was affected little by the inoculation seasons. Disease ratings of varieties showed a continuous variation with a single mode in the resistant range in each of the four isolates. However, the correlation coefficient between Japanese isolate K105 and Canadian isolate WRS102 was as low as 0.55, indicating a slight pathogenic differentiation between these isolates. Significant correlation coefficients (r=.55~.78) among the ratings of isolates indicated that the pathogenicity to the varieties was rather similar and that the pathogenic differentiation was small among the four isolates tested. In general, varieties from Ethiopia, North Africa and Korea were more resistant than those from other regions. Varieties from Turkey and Europe were susceptible to Japanese isolates, while Nepalese varieties were susceptible to Canadian isolates. Twenty of 25 varieties which were resistant to the isolate K105 but susceptible to the isolate WR102 were from Nepal and most of those were Oriental-type (Bt bt2) in brittleness of rachis. These findings revealed an example of regional concentration of resistant gene in net blotch. |
キーワード | Barley Net blotch Disease resistance Genetic resources Race differentiation |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 91 |
終了ページ | 102 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313965 |
タイトル(別表記) | Diallel Analysis for the Percentage of Grains with Hull Rupture in F2 Populations of Two-rowed Barley |
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フルテキストURL | 002_001_079_089.pdf |
著者 | 呉 基日| 武田 和義| 金谷 良一| |
抄録 | Half diallel F2 populations crossed among eight two-rowed barley varieties with warious percentages of hull ruptured grains were raised to analyze the inheritance of the trait. The percentage of grains with hull rupture varied from 0 to 87% among the parents. The diallel analysis revealed the following: the trait was predominantly controlled by the additive genes, and the dominance effect of some parents was also significant. However, the epistatic effect of the genes was not significant. The average dominance was 0.97. The heritability value was estimated as 0.57 and 0.91 in a narrow and a broad sense, respectivelly. The percentage of grains with hull rupture showed continuous and transgressive segregations in 28 F2 populations derived from half diallel crosses among eight parents. Heritability of the trait in a broad sense was 0.43~0.80(0.65 on average) in 28 F2 populations. |
キーワード | Barley Grain quality Hull rupture Diallel analysis Heritability |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 79 |
終了ページ | 89 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Host Range and Some Properties of Orchid Fleck Virus Isolated form Oriental Cymbidium in Japan |
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フルテキストURL | 003_002_151_161.pdf |
著者 | 近藤 秀樹| 松本 純一| 前田 孚憲| 井上 成信| |
抄録 | Orchid flck virus (OFV) was isolated from Oriental Cymbidium (Cymbidium sp.),showing chlorotic flecks on leaves. The virus was transmitted mechanically to Chenopodium quinoa,C.murale and Beta vulgaris by sap-inoculation and caused systemic infection. Local lesions were produced on C.amaranticolar, Petunia hybrida, Tetragonia expansa and Vigna sinensis. Sap from infected T.expansa was still infective after 10 mim at 40℃ but not after 10 min at 45℃, at a dilution of 10-3 but not 10-4, and after 30 min at room temperature but not after 60 min. The isolate of OFV had non-enveloped, bullet-shaped patricles measuring about 40×120-150 nm in dip preparations. However, bacilliform particles about 40×120-140 nm were observed in ultrathin sections. In ultrahtin sections of virus-infected tissues, virus patricles were detected both in the nuclei and in the cytoplasm. Moreover, the inclusions of low electron density(viroplasm) were also observed in the nuclei.Virus particles were found to attach at one end to the inner nuclear membrane. A number of particles surrounded by the inner membrane often showed an appearance like a spoked wheel. |
キーワード | Orchid fleck virus Oriental Cymbidium Non-enveloped rhabdovirus like particles |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 151 |
終了ページ | 161 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313940 |
タイトル(別表記) | Character Expression and Inheritance of a "Short Upper Leaves" Mutant in Barley |
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フルテキストURL | 002_001_069_078.pdf |
著者 | 金谷 良一| 呉 基日| 武田 和義| |
抄録 | A mutant which devolops an extremely shortened flag leaf and shorter upper leaves was obtained from the progeny of 90 KR of gamma-ray irradiated Fuji Nijo, a malting barley variety. This mutant was controlled by a single partial dominant gene named Sul (short upper leaves). The character expression and the inheritance of the gene were investigated in this study. Sul shortened the flag leaf and upper several leaves and uppermost internode length, while it did not affect the length of spike, lower leaves and the second or lower internodes. A reciprocal translocation of the chromosomes was observed in this mutant line, and the Sull gene was linked with the V (two-rowed) gene on chromosome 2, and also linked with the breaking point of the reciprocal translocation. Small leaf area caused by the Sull gene might reduce the mutual shading and the transpiration of the leaf canopy at the later growth stages. |
キーワード | Barley Plant type Linkage analysis Developmental pattern |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 69 |
終了ページ | 78 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Cloning of PCR-Products Encoding Potassium Channel Proteins from Mesembryanthemum crystallinum |
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フルテキストURL | 003_002_145_149.pdf |
著者 | 且原 真木| Bohnert Hans J.| |
抄録 | Gene fragments of potassium channels were cloned from Mesembryanthemum crystallinum by using RT-PCR (reverse transcription-polymerase chain reaction). The two fragments were isolated independently and showed high similarity with each other. About 80% identity was found between the two fragments and potassium-channel genes of Arabidopsis. Southern hybridization indicated that the potassium channel gene may be a single copy gene or that a small gene family of potassium channels exists. |
キーワード | Mesembryanthemum crystallinum Potassium channel RT-PCR |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 145 |
終了ページ | 149 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification |
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フルテキストURL | 003_002_137_143.pdf |
著者 | 秋山 佳子| 柴坂 三根夫| 河﨑 利夫| |
抄録 | Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged. |
キーワード | Adenylate extraction ADP AMP ATP Barley roots |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 137 |
終了ページ | 143 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Effects of Mannosen on Hydroponically Grown Barley |
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フルテキストURL | 003_002_129_135.pdf |
著者 | 柴坂 三根夫| 宮田 將彦| 秋山 佳子| 河﨑 利夫| |
抄録 | To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants. |
キーワード | Barley Hydroponics Mannose Potassium Phosphate |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 129 |
終了ページ | 135 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Genetic Analysis of Large Trichome in Barley Leaf Blade |
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フルテキストURL | 002_001_063_068.pdf |
著者 | 佐藤 和広| 武田 和義| |
抄録 | The inheritance and linkage relationship of a new hairiness trait "large trichome" was investigated in barley. Although the size of large trichome is about four times that of normal one, the character can not be recognized with the naked eye. However, it is easily identified by the roughness of leaf touch. The large trichomes develop on both sides of the leaf blades. The direction of trichome is both acropetal and basipetal. It is clearly distinguished from the extremely long trichome controlled by Pub gene. About 2,300 varieties of our Barley Germplasm Center were screened by the leaf touch to find nine varieties with large trichome. Two of them were six-rowed local variety from Pakistan, and other seven were two-rowed varieties from Europe and Japan. All of them were hulled type. Crosses of six large trichome varieties with a normal Japanese variety resulted in the large trichome type F1s, suggesting the dominant nature of the trait. The large trichome line Hokuiku 17 was crossed with various linkage testres to study the mode of inheritance and the linkage relationship of the gene. In the F2 populations, the large trichome was controlled by a single dominant gene named Ltr (large trichome), which was independentiy inherited from the following marker genes; br and gl-5 on chromosome 1; li and ν on chromosome 2; uz on chromosome 3; K and gl-3 on chromosome 4; trd on chromosome 5; ο on chromosome 6. On the other hand, from the cross between Hokuiku 17 and OUL166, Ltr was found to be linked with s and fs on chromosome 7. Although the allelism test has not been completed, the very low frequency of the large trichome type (9/2,300) indicates that the variant resulted from a recent mutation event, or the fitness of the variant is low in the natural and/or artificial selection. |
キーワード | Barley Trichome Linkage analysis |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 63 |
終了ページ | 68 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313663 |
タイトル(別表記) | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
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フルテキストURL | 003_002_117_127.pdf |
著者 | 米谷 力| 森次 益三| 河﨑 利夫| |
抄録 | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
キーワード | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 117 |
終了ページ | 127 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Inheritance of Phenol Reaction in the Awn of Barley |
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フルテキストURL | 002_001_055_062.pdf |
著者 | 武田 和義| 張 成林| 金谷 良市| |
抄録 | The inheritance and geographical distribution of the phenol reaction in rice has been investigated intensively. On the other hand, in the case of barley, almost all of the varieties show positive reaction to phenol, and inheritance study of the trait has not yet been reported. We investigated the phenol reaction of ca. 5,000 barley varieties preserved at the Barley Germplasm Center of Okayama University to find about 30 varieties which show a negative reaction to the phenol. These varieties are mainly from Southwest Asia. The reaction of awn was sharpest at all parts of the plant including seeds. The positive reaction must be the prototype of barley, because Hordeum spontaneum, a possible ancestor of the cultivated barley, shows positive reaction. Inheritance and linkage studies were conducted using varieties with negative reaction and linkage testers with positive reaction. Phenol reaction was dominant and showed a clear 3:1 segregation ratio in the F2 populations, indicating the trait was controlled by a single dominant gene Ph(phenol reaction). Linkage study revealed that Ph was linked with four marker genes(three loci) on the chromosome 2 and independent of 13 other marker genes located on the chromosomes except chromosome 2. Ph may be a useful marker gene and an interesting material for molecular-biological studies. |
キーワード | Barley Phenol reaction Linkage analysis |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 55 |
終了ページ | 62 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Effect of Light Irradiation on the Absorption of Rubidium in Chlorella |
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フルテキストURL | 003_002_105_116.pdf |
著者 | 蜂谷 欽司| 森次 益三| 河﨑 利夫| |
抄録 | The effect of light irradiation on the absorption of rubidium was examined in Chlorella. Rubidium absorption in Chlorella was clearly stimulated by the irradiation of light. To clarify the mechanism of light-stimulation on rubidium absorption, experiments were carried out using several metabolic inhibitors; DCMU, NaCN,DNP,CCCP and ouabain. Among the metabolic inhibitors used, DCMU had the most similar effects on the rubidium absorption and oxygen evolution under light condition. These findings suggest a close correlation between the light-stimulation on rubidium absorption and the photosynthetic process in Chlorella. |
キーワード | Chlorella Light-stimulation Metabolic inhibitors Oxygen evolution Rubidium absorption |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 105 |
終了ページ | 116 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Genetical Studies on Callus Growth and Plant Regeneration from Immature Embryos in Barley |
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フルテキストURL | 002_001_043_053.pdf |
著者 | 間野 吉郎| 力石 和英| 安田 昭三| |
抄録 | Immature embryos of 99 varieties of barley were cultured to investigate the ability of callus growth and plant regeneration. These two in vitro traits showed wide and continuous variations among the barley varieties tested. Ability of callus growth, which were evaluated by callus diameter ranged from 3.9mm to 11.2mm, and ability of plant regeneration from the calli ranged from 0% to 100%. A set of complete diallel crosses was made using six cultivars as the parents which differed in ability of callus growth and plant regeneration. The Vr/Wr graphical analysis showed that there were epistasis, or interaction among nonallelic genes for callus growth. As to ability of plant regeneration, no epistasis existed in the subdiallel without P1 (J232) which showed high specific combining ability, and it was controlled by a simple additive dominance genetic system. The mean degree of dominance(0.42) was relatively low and the broad(0.86) and narrow(0.78) sense heritabilities were high. |
キーワード | Barley Tissue culture Plant regeneration Diallel analysis |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 43 |
終了ページ | 53 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | 数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 |
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フルテキストURL | 003_002_091_103.pdf |
著者 | 森次 益三| 河﨑 利夫| 鈴木 孝夫| |
抄録 | Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. |
キーワード | Alkaline earth Ammonium Ion balance Nitrate Phosphate |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 091 |
終了ページ | 103 |
ISSN | 0916-930X |
言語 | 英語 |
論文のバージョン | publisher |
タイトル(別表記) | Comparison of Regenerating Ability of Calli Derived from Mature and Immature Embryos in Barley Varieties |
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フルテキストURL | 002_001_033_042.pdf |
著者 | 力石 和英| 安田 昭三| |
抄録 | The callus forming ability and regenerating ability of the calli derived from mature and immature embryos of 132 barley varieties were examined. These materials were taken from a world-wide collection preserved at the Barley Germplasm Center of Okayama University. The callus forming ability varied widely according to genotype in both mature and immature embryos, but the varieties collected from Ethiopia showed low callus forming ability. Calli derived from mature embryos generally did not regenerate shoots, except for three Japanese varieties. The frequency of shoot regeneration from the calli derived from immature embryos was somewhat higher than that from those derived from mature embryos. Many of the Korean and Japanese varieties had a high shoot regenerating ability. However, few of the varieties from Ethiopia and Southwest Asia had a high shoot regenerating ability. No correlation was observed btween root regenerating ability and shoot regenerating ability of the varieties. No correlation was observed between callus proliferation and root regenerating ability between calli derived from mature and immature embryos. We could not find any difference in the shoot regenerating ability btween the two-rowed and six-rowed genotypes. |
キーワード | Barley Tissue culture Mature embryo Immature embryo Regenerating |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 33 |
終了ページ | 42 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Promotion of Drying of Leaves Detached from Plants after Rainfall Exposure |
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フルテキストURL | 002_002_191_200.pdf |
著者 | 木村 和義| |
抄録 | The drying of leaves detached from 16 plants (mainly, vegetalbles) after artificial rainfall (mist)treatment was examined in a growth chamber(20℃,8 klux). Leaves detached from misted plants had a higher drying rate than those detached from non-misted plants. The promoting effect of mist on the dryint rate was increased with the increase in the duration of mist exposure. The degree of drying after mist exposure for 3 days or more was increased markedly. In almost all the plants,the weight of the leaves detached from the plants decreased to 10-20% of the initial weight 24 hours after a 5-day mist treatment, but 40-50% in cabbadge, chinese leek and welsh onion. In general, the degree of drying was greater in the young developing leaves or older leaves than in active young leaves. These findings suggested that the surface wax and cuticular of the leaf is injured by rainfall wetting, and that water discharge(transpitation) in the leaf is increased greatly. |
キーワード | Rainfall Leaf Drying Vegetables |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 2号 |
開始ページ | 191 |
終了ページ | 200 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Varietal Variation of Earliness in a Narrow Sense and Its Significance for Adaptation in Barley |
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フルテキストURL | 002_001_023_032.pdf |
著者 | 大久保 和男| 安田 昭三| 武田 和義| |
抄録 | Heading time of is a complex character, which is controlled by several internal factors, namely, vernalization requirement, photoperiodic response and earliness in a narrow sense (ENS). The ecological significance of vernalization requirement and photoperiodic response has been clarified. However, the significance of ENS is not explained well. In this study, we examined the varietal variation of ENS, its geographical distribution and significance of ENS for regional adaptability in barley. The ENS was estimated from the number of days from sowing to flag-leaf emergence of sufficiently vernalized plants grown under a 24-h. photoperiod at 20℃ condition. The variation of ENS was determined from the number of leaves and leaf emergence intervals under the controlled condition. The ENS of 1,017 barley varieties ranged from 12 to 28 days. ENS was shortest in varieties from low-latitudes, while it was longer in varieties from high-latitudes and very low-latitudes. Multiple regression analysis for heading time(Y) on the ENS(X1) and the photoperiodic response(X2) was carried out using various data obtained from different experiment sites and sowing times. The multiple correlation was highly significant in all cases. It was clear that the ENS played an important role for determining heading time in the spring-sown conditions, while the photoperiodic response did in the fall-sown condition. |
キーワード | Barley Heading time Geographica distribution Adaptability |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 23 |
終了ページ | 32 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | タバコ懸濁培養細胞におけるアルミニウムの二価鉄、銅およびカドミウム毒性に対する影響 |
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フルテキストURL | 002_002_181_190.pdf |
著者 | 山本 洋子| 張 藝潔| 小野 寛治| 松本 英明| |
抄録 | The effects of aluminum (Al) on the cytotoxicity of ferrous iron (Fe(Ⅱ)), copper(Cu) and cadmium(Cd) were studied. Log-phase cells were treated with either FeSO4,CuSO4, or CdCl2 in the presence or absence of AlCl3(120μM) for 18h at pH 4.0. After the treatment, the viability was determined as relative growth of the metal-treated cells to the untreated control cells during the post-treated culture. A single treated with either Al, Fe(Ⅱ) or Cd did not inhibit the growth at the metal concerntrations up to 300 μM, 200 μM and 500 μM, respectively, whereas the growth was markedly inhibited at 15 μM Cu. Thus,the cells were relatively insensitive to Al, Fe(Ⅱ) and Cd and sensitive to Cu. When cells were treated with both Fe(Ⅱ)(120 μM)and Al(120μM), the growth was significantly inhibited and the cellular contents of both Al and Fe increased synergistically. After the treatment with Cu(0 to 10 μM) with or without Al, the cells grew more vigorously when they were treated in the presence of Al, althrouh the Cu content of the cells were not alterd by Al. The presence of Al during the treatmemt with Cd(0 to 2 μM) had no effect on the degree of growth inhibition by Cd. Thus, Al interacts with the toxicity of Fe(Ⅱ), Cu and Cd in different manners; synergistic with Fe(Ⅱ), antagonistic with Cu and apparently no effeco on Cd. |
キーワード | Aluminum Antagonistic Nicotiana tabacum Suspension culture Synergistic |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 2号 |
開始ページ | 181 |
終了ページ | 190 |
ISSN | 0916-930X |
言語 | 英語 |
論文のバージョン | publisher |
タイトル(別表記) | Studies on Heterosis in Barley Cultivars |
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フルテキストURL | 002_001_007_022.pdf |
著者 | 安田 昭三| 林 二郎| 守屋 勇| |
抄録 | In cultivated barley, various hybrid injuries such as brittle rachis and hybrid weakness occur. Therefore, crossing must be done among cultivars to prevent the occurrence of such hybrid injuries. In the present study, we examined the magnitude of the heterosis in the crossings and geographical distribution of barley cultivars concerning heterosis. A few Japanese cultivars were used as the common parent for crossing. (1) In the six-rowed barley, 43 cultivars collected from Japan, China and Korea were crossed with non-uzu covered and naked cultivars, Shiroyo-shigara 2 and Nami-Akashinriki, respectively. The largest magnitude of heterosis in grain yield per plant was obtained in the crossing between the Korean cultivar and one of the Japanese common parents. (2) Heterosis in two-rowed cultivars was investigated using a total of 156 F1S which were cross combinations of cultivars collected from the world with two common male parents, Amagi Nijo and Kawasaigoku. The heterosis was larger larger in the cross combinations with Turkish and Ethiopian cultivars. The largest F1/BP was 1.66 in Turkish and 1.45 in Ethiopian cultivars. (3) As to uzu or semi-dwarf cultivars peculiar to Japan, 34 cultivars were crossed with two common male parents, Sekitori (covered) and Akashinriki (naked). Expression of the heterosis was rather conspicuous in the crosses of covered×naked cultivars than those of covered×covered and naked×naked ones. (4) Half diallel analysis using 10 two-rowed cultivars showed that one Ethiopian cultivar expressed a high grade of general combining ability and that one French cultivar was of higher specific combining ability. (5) The most effective agronomic character of the two-rowed cultivars to the grain yield per piant was number of ears in an ordinary year, while in the wet year it was 1000 grain weight. This was considered to be due to overluxuriant growth accompanied by lowering of the percentage of ripened grain. However, in some cultivars the percentage of ripened grain was not lowered under the overluxuriant growth condition. |
キーワード | Barley Heterosis Diallel analysis Overluxuriant growth Geographical variety |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 1号 |
開始ページ | 7 |
終了ページ | 22 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | ゼニゴケ葉緑体遺伝子と相同性を持つラン藻 Synechocystis PCC6803株のORF326、frxC およびORF469を標的にした変異の導入 |
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フルテキストURL | 002_002_167_180.pdf |
著者 | 小倉 豊| 竹村 美保| 小田 賢司| 大山 莞爾| |
抄録 | ORF326, frxC and ORF469 of a transfomable cynobacterium, Synechcystis PCC6803, have sequence similarity with ORF465 on the choroplast genome of a livewort, Marchantia polymorpha, respectively. To elucidate their functions,targeted mutagenesis was performed by transformation with clened DNA in which the ORF was disrupted by insertion of a kanamycin resistancen gene cassette.Streak-purifications of a single colony of each transformant were repeatde to segregate homozygous mutants for disrupted copies, because Synechocystis PCC6803 was reported to have approximately 10 chromosomal DNA copies. Southern blot analysis revealed that mutants for ORF326 had not only disrupted ORF326 copies but also wild type ORF326 copies. This suggests that ORF326 is indispensable for growth under the mixotrophic growth condition used. However, mutants for frxC and mutants for ORF469 had only mutated copies, indicating that they dispensable for growth. Growth and chlorophyll a content of an ORF469-disrupted mutant were compared and chlorophyll a content of an ORF469-disrupted mutant were compared to those of wild type under mixotrophic growth condition, but no significant difference was detected. This indicates that ORF469 is required for neither normal growth nor chlorophyll biosynthesis under thie condition. |
キーワード | Cyanobacteria Gene disruption Synechocystis PCC6803 |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1994 |
巻 | 2巻 |
号 | 2号 |
開始ページ | 167 |
終了ページ | 180 |
ISSN | 0916-930X |
言語 | 英語 |
論文のバージョン | publisher |
著者 | 米谷 俊彦| |
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発行日 | 1994 |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
巻 | 2巻 |
号 | 2号 |
資料タイプ | 紀要論文 |