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Die Autoren haben 48 Patienten mit chronischer Koronarinsuffizienz mit Nitrolingual Retard Kapseln bettlagerig oder ambulant behandelt. Nach ihren Ergebnissen kann man sagen, dass Nitroglycerin in einer Langzeitform als Nitrolingual Retard Kapsel den modernen Erfordenissen der Therapie der Koronarinsufflzienz entspricht, da damit iiber einem langeren Zeitraum hinweg gleichmassig Blutspiegelwerte erzielt werden konnen. So hat man einen guten therapeutischen Erfolg, dazu einen Riickgang der Anfallhaufigkeit und eine erhohte Belastbarkeit erreicht. Der Wirkstoff hat iiber einem Zeitraum von 10-12 Stunden sich konti. nuirlich diffundiert bzw. klinisch gewirkt. Bei der Einnahme von Nitro. lingual Retard sind keine Kopfschmerzen aufgetreten. Die Vertraglichkeit des Praparateserwies sich als hut.

A non.specific esterase activity was demonstrated III the jejunum of rats by an azoindoxyl method. 1) Microvilli of the jejunal epithelial cells were remarkably stained in non-frozen specimens and feebly in frozen specimens. 2) The other cytoplasmic structures, i. e. mitochondria, endoplasmic reticulum, nuclear envelope, plasma membrane and multivesicular body showed a positive reaction product in frozen sections but not in non-frozen blocks.

A human fetal fibroblast strain, belonging to a group resistant to SV40 transformation, was transformed by SV40 through a multiple inoculation procedure. Two independently transformed cells were described in comparison with each other. The proportions of cells with the nuclei possessing V antigen were 2.9% at the 5th passage in one strain and 1.1 % at the 4th passage in another, and they declined gradually as frequent passages were repeated. The percentages of the transformed cells with V antigen-positive nuclei were, in both strains, quantitatively compatible with those of the cells with the nuclei full of virus particles in crystalline arrays, which were demonstrated by immunofluorescent studies and electron microscopy.

An electron microscopic observation was made on the DNA's extracted from purified HeLa cell nuclei, mitochondria, and the whole cell, and fractionated by ethidium bromide-cesium chloride density gradient method or sucrose density gradient method. Nuclear DNA presents mainly long linear DNA derived from fragmented chromosomal DNA. In addition to this, the existence of small circular DNA molecules measuring 0.32 -1.78 μ, was confirmed. Mitochondrial DNA was mainly circular DNA, which measured 4.87 μ in the mean value of the contour lengths in the highest frequency group, and small circular DNA molecules, measuring 0.3-1.01 μ in contour length, were also found in an extremely low frequency.

Two cases of multiple foreign bodies, i. e., duplex coins in the esophagus are reported. These foreign bodies were removed by peroral esophagoscopy successfully. Significance of roentgen-ray diagnosis is emphasized, and subtle and yet specific roentgenograms of duplex coins in the esophagus are illustrated.

Die stark eisenhaltigen Praparate Ferrlecit, Kobaltjerrlecit, Ferrojolsan, Ferrojolcamma und Irradian wurden im Siemens Unterrichtsreaktor SUR 100 des Instituts fur Kernenergetikder Universitat Struttgart und der Technischen Universitat, Berlin mit einem NeutronenfluB von ca. 5.l06n/cm2 sec bestrahlt. Es ollte dabei untersucht werden, wie stark eisenhaltige Praparate mit diesem Reaktor aktiviert werden konnen. Es wird festgestellt, daB Eisen praktisch kaum aktiviert wird. Die Ergebnisse zeigen, daB nur das Kobattjerrlecit und das Irradian starker durch Neutrosen aktiviert werden. Die hohere Aktivitat des Kobaltjerrlecit wird auf die darin enthaltenen Elemente Mangan, Kupfer und Kobalt zuruckgefuhrt, die mit thermischen Neutronen gut aktivierbar sind. Die hohere Aktivitat des Irradian geht hauptsachlich auf β-Zerfalle zuruck. Das Phosphorisotop P-32 tragt einen groβen Anteil dazu bei. De rβ-Untergrund des Irradian bei hohen Energien, der mit einer kleinen Halbwertszeitabklingt, ist durch keines der angefuhrten Elemente im Irradian erklarbar. Der Mn-56Peak im Spektrum ist gut erkennbar, durch den β-Untergrund wird er aber zu einer hoheren Energie hin verschoben.

By assuming a three-compartment model, kinetic analysis of peripheral hyroxine distributions in various organs was represented by the alues calculated on the basis of the disappearance curve of 131I-T4 radioactivity in the serum, time　dependent curve of radioactivity over the liver　and urinary excretion of 131I-T4 in attempts to clarify the kinetic distribution　of the thyroxine and the time　dependent pool size of thyroxine in each　compartment such as serum pool, liver pool, and the other pool,　As a result it has been demonstrated that pool size3 of tyroxine, in the serum, liver and the other pool are enlarged in　hyperthyroidism, while　they are decreased in　hypothyroidism in respective pools.　We have recognized the reduction in the values of the liver pool size　of 131I-thyroxine and 131I-thyroxine excretion into the bile, while the increase　of 131I-thyroxine excretion into the urine in the cases of chronic hepatitis　and cirrhosis of the liver, but the thyroxine concentration in the serum to remain within the normal level in liver diseases. As a result of the reduction　in the liver pool size of 131I-thyroxine and in its uptake into the liver,　the other pool size enlarges to compensate the reduction and the function of the other pool is elevated.

As a step in the elucidation of immunity of human cancer from the standpoint of homotransplantation immunity, we conducted mixed cultures of regional lymph node cells from C3H mouse isotransplanted with methylcholanthrene-induced sarcoma (MC-tumor) together with the primary culture MC-tumor cells, and observed the behaviors of these lymph node cells to the MC-tumor cells and compared the effects of these lymph node cells with lhose of normal mouse lymph node cells by counting the growth number of tumor cells and also by cinematography. As a result, it has been demonstrated that the regional lymph node cells from the mouse isotransplanted with the MC-tumor (2mm3 in size) acquire a strong antitumor activity by 14 days after the transplantation, but such antitumor activity diminishes and disappears in the terminal stage of cancer. When the number of these lymphocytes is increased, there can be observed some dosage effect, but no complete inhibition of the tumor growth can be attained. The cinematographic observations of these regional lymph node cells in the mixed culture with tumor cells reveal that lymphocytes of small to intermediate size aggregate onto the tumor cells and inhibit the movement of the latter.

The present report describes the findings on the infectivity of DNA partially purified from SV-40 which was propagated in the monkey kidney cells (BSC-1) in vitro and the importance of nucleic acids as oncogenic factors, particularly the induction of tumor by DNA in newborn hamsters. 593 newborn hamsters in total were used in the present experiments, and cannibalism among them posed as a serious problem. On 30 days postinoculation, very remarkable changes occurred in the liver, lung and subcutaneous areas. Cellular responses of the perivascular cells were predominant. and they were distributed in the interstitial tissues of the liver (liver cirrhosis in primates) and lung. Three hamsters of those subcutaneously inoculated with nucleic acids developed tumors and two tumors appeared in the subcutaneous tissues on 130 days postinoculation, which were identified to be the ones induced by intact SV-40 virus. Other tumors appeared in the liver, lung, intestinal ducts and abdominal surface at 126 days after subcutaneous injection. The cytological observations revealed multiple hemangiosarcoma combined with proliferation of the perivascular cells. On the other hand, cellular responses to nucleic acids were more marked by inoculation of the cell-free filtrate of BSC-1 infected by DNA than of DNA, and essential histologic findings were similar to the respo.nse to infectious DNA. Thirty-nine hamsters (30 per cent) developed tumor within about 200 days postinoculation of the filtrates. Sarcomas were common and they were confined to the subcutaneous tissues in 35 hamsters and to the peritoneum in two others by subcutaneous inoculation of the filtrates. The intestinal gland-cell carcinomas, however, could be induced at 37 and 59 days postinoculation in two hamsters of one litter (7 newborn hamsters) and in the other three newborn hamsters subcutaneous sarcomas were induced by inoculation of the same agent. These results suggest that the observation on the oncogenic capacity of nucleic acids would give us a clue to resolve the course of cancer from the view point of the infectious nucleic acid.

The distribution and activities of five hydrolytic and eight oxidative enzymes were histochemically studied in 60 different tumors of the human intestines. Benign polyp showed similar activities of most enzymes as those in normal crypt cells of large intestine with exception of higher activity of succinic dehydrogenase in benign polyp than in crypt cells. Malignant polyp had higher activities of most oxidative enzymes. Reticulo-sarcoma had weak activities of all enzymes. Carcinoid had strong activities of glucose-6-phosphate dehydrogenase and isocitric dehydrogenase while very weak of succinic dehydrogenase. Carcinoma showed varying degrees of the activity of all enzymes. Alkaline phosphatase and aminopeptidase were almost negative in all cells but in the stromal elements their weak activities were sporadically observed. Most enzymes were decreased in the central area of the carcinoma cell nestle, while in the infiltrating area or in the margin of cell nestle they were not decreased and sometimes increased.

1. The ratios of free 5α-cholestan-3β-ol and cholesterol and esterified 5α-cholestan-3β-ol were higher in pylorus than in cardia. 2. Esterified cholesterol level was higher in cardia than in pylorus. 3. Among the stomach cancer tissues examined free cholesterol level was higher than in the non-cancerous. 4. Esterified 5α-cholestan-3β-o1 and cholesterol levels were lower in the cancerous tissues than in the non cancerous.

1. Isovaleric acid-1-C14, -4-C14, or C14-CaC03 with or without non-isotopic isovaleric acid was orally administered to rats and the incorporation of these isotopes into liver cholesterol, fatty acid, or urinary isovalthine was examined. 2. Isopropyl group of isovaleric acid was more efficiently utilized for cholesterol synthesis than carboxyl group, and also for cholesterol synthesis than for fatty acid. These results indicate that isovaleric acid is cleaved into two fragments before it is utilized for cholesterol synthesis. 3. Carbon dioxide was used for the synthesis of liver cholesterol and of liver fatty acid. Isovaleric acid seems to enhance the incorporation of carbon dioxide into cholesterol. 4. All the experimental rats received isotopic or non-isotopic isovaleric acid excreted isovalthine, but no radioactivity was found in it. Thus, isovaleric acid residue of urinary isovalthine molecule is not derived from isovaleric acid administered, and carbon dioxide is not the carbon source of urinary isovalthine. 5. Suspicious metabolism of isovaleric acid or of carbon dioxide was discussed. 6. Isotopic isovalthine which was synthesized from (± ) α-bromoisovaleric acid-4-C14 is administered to rat and it was found that the isotope did not incorporate into cholesterol or fatty acid of liver and of brain. About 15% of isotopic isovalthine was recovered in urine up to the next day after injection. The large part of isovalthine was missing.

1) Three different types of muscle fibers were clearly distinguished in the intercostal muscles and the diaphragm of human, cat and rat by histochemical demonstration of oxidative enzymes, phosphorylase and glycogen. 2) The intercostal muscles and diaphragm each presented dissimilar patterns of the muscle fibers. The diaphragm did not show any definite correlation between the diameter and the histochemical reaction of muscle fibers but its red fibers indicated a more intense uptake of Nitro-BT formazan deposits in the subsarcolemmal region. In this conection, the relationship between the motor innervation and histochemical evaluation of the diaphragm was described. 3) Phosphorylase and glycogen showed reciprocal reactions to the oxidative enzyme activity. They were generally high in large fibers but low in small fibers, and moderate in intermediate fibers.

With gastric carcinomas the activities of eight dehydrogenases; succmlC, lactic, malic, α glycerophosphate, glutamic, β-hydroxybutyric, glucose-6-phosphate and isocitric dehydrogenase were statistically estimated. Principal findings may be briefly summarized as follows. These enzymatic activities differed considerably even in the same classification of carcinomas and generally ranged from strong to weak in the following order: lactic, malic, glucose-6-phosphate, isocitric, succinic, α-glycerophosphate, glutamic and β-hydroxybutyric dehydrogenase. The activities of adenocarcinomas were stronger than those in simple ones, and these were not related appreciably to cell differentiation in adenocarcinomas except succinic, glutamic, glucose-6-phosphate and isocitric dehydrogenase. As for succinic dehydrogenase and NAD-linked dehydrogenases except for lactic dehydrogenase, the activities were strongest in intestinal metaplasia and early mucosal carcinomas, the next being in benign adenomatous polyps and weakest in the other carcinomas. As for NADP-linked dehydrogenases and lactic dehydronase, the activities were also strongest in intestinal metaplasia and early carcinomas, the second in the other carcinomas and the third in the benign polyps. Generally, these dehydrogenase activities were strongest in free carcinoma cells in blood and lymph vessels and in actively growing part of several carcinomas and weakest in the central area of tumors, especially almost negative in the central necrotic area.

Summing up the above problems we may group them as follows: 1) Szirmai's angio-myograph and myotonometer furnish us with means to evaluate the author's successful method or medical treatment in thrombophlebitic, postthromboembolic (ulcer, etc···) states on the basis of the blood circulation through the muscles, clearly registered by the angio-myograph. 2) Szirmai's medical preparation "HAH" serves as a quick and effective cure for thrombophlebitis. Results are very often reached within a few days. The patient's health is restored so as to make him able to work. 3) The above preparation assures full success in the cure of thrombotic esp. thromboembolic states of the lower limbs-cases of ulcus cruris includedwhich up to now could not be favourably influenced by any other method of treatment. Description of varieties of above problems and other types of cases of peripheral circulation (Endangitis, etc.) and their relationships with the subject will be given in additional papers. The author reports on registering and controlling thrombophlebitis, postthromboembolic states, including ulcus cruris, origin~ting either in above morbid conditions or in independent causes by means of the angio-myograph and myotonometer devised by the author. The reader is made familiar with the author's (Szirmai's) preparation "HAH" (Heacrin) and with the results achieved by applying it for the cure of acute thrombophlebitis and thrombotic states. Results are often showing up remarkably soon (2 to 6 days).

Les auteurs out effectue après une irradiation totale de 1200r de rats blancs des deux sexes des examens hématologiques à la suite d'irradiations ainsi que des examens physiologiques et des contrôles. Ils n'ont observe de modification importante des facteurs coagulants qu'au troisieme jour; cette modification était maximum avant la mort, c'est-à-dire au stade terminal. Les temps de coagulation naturelle ont beaucoup diminué, de même que ceux de la thrombine et ceux de la thrombine avec le bleu de toluidine, c'est-à-dire que l'héparine libérée ( = antithrombine semblable à l'héparine) a diminue. Pour les facteurs V et VII et en particulier pour la prothrombine on a observe un fort accroissement de la concentration. Les auteurs pensent que ceci est explicable par le fait que la décomposition des tissus pendant l'irradiation entraine la libération de kinase et d'autres activateurs dans la circulation sanguine, ce qui provoque une anoxemie des tissus. D'autres expériences sont en cours en collaboration avec de nombreux spécialistes et instituts.

,The effect of infection of human embryonic skin-muscle cell cultures with adenovirus type 12 has been studied. When maintained in YLE containing 20 per cent bovine serum, human embryonic skin-muscle tissue culture cells developed little or no cytopathogenic effect for about 50 days after inoculation of adenovirus type 12, though a small amount of virus was always detected in the overlying medium. From day 50∼60, CPE started appearing and spread over 90 per cent of cells accompanied with the increase of virus in the overlying medium. The addition of human serum to the maintenance medium inhibited the virus release. After removal of human serum about 16∼37 days after its addition, virus-and, later, CPE also-again started appearing. The second virus release-and CPE also-was inhibited by addition of human serum to the medium. When maintained in the medium with human serum for about 200 days, the removal of human serum did not result in the appearance of virus or CPE. The virus isolated from the overlying medium of these cells during the whole process of the experiment was always highly oncogenic to newborn hamsters. Diluted adenovirus-12-immune rabbit serum also showed the effect similar to that of human serum. But, regardless of its much higher antibody titer, the effect of this diluted adenovirus-12-immune rabbit serum was weaker than that of human serum. In one of cell cultures, rapidly growing cells appeared 212 days after virus inoculation. But the available data suggest that these are the cells transformed rather spontaneously in tissue culture than by adenovirus type 12.

In the present experiment, it has been noted that clonizing epithelial-like cells of the intestine 407 were more susceptible to SV-40 virus than normal fibroblasts in primary human cell cultures. In the early stage of the infection the cell growth was enhanced by the inoculation of DNA virus but many cells died, showing lysis characterized by CPE, clumping of chromatin and formation of inclusion bodies. On the other hand, the cells surviving infection have given rise to virus-free long term cultures and cellular responses to the virus characterized by cell proliferation which is. classified in four phases. (Phase. I: infection and cell alteration. Phase. II: crisis. Phase. III: fibro-reticulum cell formation. Phase. IV: recovery and proliferation). The most remarkable morphological characteristic was fibroblastic cell alteration from epithelial cells at 5 weeks of virus inoculation. By this study an interesting generalization of human epithelial-like cells can be made about the differentiation of the transformed cells in relation to SV-40 virus and it has been shown that an established human cell line is still susceptible to the reverting action of the SV-40 virus.

Chromatography on Sephadex G-200 was performed with the soluble fraction of homogenated rabbit liver, which was extracted with 0.1 M phosphate buffer containing 0.1 M NaCl. and the influences of autolysis on the soluble fraction of liver were also examined. The soluble fraction of liver was different from serum in molecular weight, in electrophoretic character and in components with sedimentation coefficients. The soluble fraction of liver was stable under the influence of Mg and K ions, and rather unstable in the presence of Na ions. Serum was fractionated in three main peaks. The soluble fraction of liver was fractionated in a similar pattern as of serum, but the first peak contained nucleic acid and lipoprotein. The second contained albumin. 32p radioactivity peaks of the stored sample appeared with change in patterns by autolysis from the original, and were observed wide based and continuous figures in retarded peaks. The correlations with the first peak and retarded peaks were represented by the analysis of phosphorus compounds and electrophoresis. In lipid analysis, both diglyceride and monoglyceride gradually decreased, and phospholipid pattern was observed to increase in retarded peaks by autolysis. Lipoprotein or lipid-albumin complex was gradually converted to smaller molecular weight compounds, and appeared in retarded peaks.

For the purpose to clarify whether minimal catalatic activity exists in Japanese acatalasemic cells or not and the manner how extrinsic hydrogen peroxide affects the acatalasemic cells, the author performed tissue cultures using the skin specimens from four acatalasemic persons affected with Takahara's disease and studied the nature of these cultured cells. The results are summarized as follows. 1. Between normal and acatalasemic cultured cells, no morphological differences could be seen and the growth rate of these cell-lines was similar to one another. 2. On the activity of succinoxidase and cytochrome oxidase there could be observed no difference between normal and acatalasemic cells. 3. In each acatalasemic cell line the minimal catalatic activity was observed and it seemed that this activity has an important role in decomposing hydrogen peroxide under normal metabolic pathway. 4. After treating with 10-4M hydrogen peroxide, respiratory enzyme activities and the growth rate in the acatalasemic cells were markedly disturbed, while in normal cells these remained almost intact. 5. There could be observed no differences between normal and acatalasemic cultured cells after X-ray irradiation (200 to 600 r) on the succinoxidase activity, catalatic activity and growth rate.