Selective staining of cytoplasmic membrane and nuclear apparatus of bacteria

A selective and simultaneous staining method for nuclear apparatus and cytoplasmic membrane of some bacteria has been presented. Nuclear apparatus is stained with basic fuchsin after hydrolysis with I N HCl and cytoplasmic membrane is restained with Victoria blue 4R after treating with saturated mercuric chloride. By this method, the nuclear apparatuses of B. subtilis, Sal. typhi 57 and Staph. aureus were stained red, and the cytoplasmic membrane and septum bluish purple distinctly. Thus this staining method would be of a great advantage in displaying the cellular structures of the bacteria.