Studies on the compounds lipids from X-ray irradiated animal,1.

For the purpose to reveal the mechanism of the biological indirect action of X-rays the author has conducted the analysis of the compound lipids of the organs of the rabbits irradiated with X-rays. Silicic acid column chromatography was applied for the fractionation of the compound lipids. Component of each fraction was analyzed by silicic acid-impregnated paper chromatography and infra-red spectrophotometory. The result proved that the compound lipids showed some qualitative and 10+-+ quantitative changes. The changes occurred mainly in glycerophosphatides showing the formation of high level lyso-phosphatidylcholine and lysophosphatidylethanolamine. The fatty acid compositions of the compound lipids were determined by means of gas-liquid chromatography. Further, polyunsaturated fatty acids were analyzed as fatty acid bromide. Major differences found in fatty acid patterns included: in the irradiated group the amount of C18-monoenoic, C18-dienoic, C18-trienoic, C20-tetraenoic and C22-acids were decreased and of C18- and C18-monoenoic acids were increased. It has been elucidated that lysophosphatides not only posesses a strong hemolytic power but also it has an action to induce swelling of the rat liver mitochondria. And it has been suggested that the lysophosphatides and ethanolamine will be responsible, at least partially, for the cell damage induced by X-rays.