Verdohemochromeの生成とその性状に関する研究 第1編 Pyridine hemin-Hydrazine-O(2)反応系によるVerdohemochromeの生成に関する分光化学的研究

The influence to the formation of verdohemochrme was spectrochemically observed on the action of hydrazine sulphate and oxygen to pyridine hemin in various changes of the reacting condition and the formation process of the product with the absorption maximum at 630 mμ was spectrochemically observed on the action of hydrogen peroxide and hydrazine sulphate to pyridine hemin under the airless condition. And the results were as follows. 1. In this reaction system, the influence of pH was great, the formation dosis of verdohemochrome was low on the high pH-value of reaction solution, the reaction was slow and the formation dosis of verdohemochrome was declined on the pH-value around 7.0, and it was decided that the pH-value at 8.5-8.0 was the most suitable. 2. The pyridine concentraticn was the most suitable in 20% of absolute concentration, the reacting process was not good below 20% and the decomposition of verdohemochrome was promoted on the over dosis. 3. There was the correlation between the hemin concentration and hydrazine concentration, the most proper concentration rate was needed and the dosis of hydrazine sulphate was needed in 10 or 25 times' Mol. concentration of hemin dosis. 4. The most proper concentration of hemin was 20mg/dl, when the concentration of hydrazine sulphate was made to be the most proper concentration rate to the hemin concentration. 5. In the reaction using hydrazine sulphate, the shaking of flask at 50°C was necessary and it was more slow in comparison with the reaction using ascorbic acid. 6. The formation of the product with the absorption maximum at 630 mμ was certified in the pyridine hemin-hydrazine-H(2)O(2) reaction system and the absorption maximum was easily shifted to 650mμ on the aeration of oxygen to the above product. 7. In this reaction system, it persevered the absorption of pyridine hemichrome, and the absorption picture of pyridine hemochrome was observed on the progression of the pyridine hemin-hydrazine-H(2)O(2) reaction system under the airless condition. 8. Since the above results, it was understood that the oxydation-reduction potential in the hydrazine reaction system was much different in comparison with that of the 1-ascorbic acid reaction system.