悪性腫瘍とcollagenase 第1編 悪性腫瘍のcollagenase活性について

腫瘍のcollagenase活性を解明する目的で,培養癌細胞および培養線維芽細胞のcollagenase活性を測定し,培養癌細胞は著しいcollagenase産生能を有するが,培養線維芽細胞には, collagenase活性はわずかであり,活性型collagenaseは,ほとんど認めなかった.さらにGross-Lapiereの組織培養法により,人正常組織,良性腫瘍,悪性腫瘍のcollagenase活性を測定し,それぞれの活性を比較検討するに,悪性腫瘍のcollagenase活性は正常組織や良性腫瘍に比較して,はるかに高活性であった.そして,これらのcollagenase活性は,動物性collagenaseによるものであることをdisc電気泳動で証明した.

The collagenolytic activity had been found associated with both epithelial and mesenchymal tumors. But it had been difficult to ascertain whether the collagenolytic activity found in primary cultures of tumors originated from the tumor cells or from host cells in reaction to the tumor. As the first step to clarify this question this experiment was designed to prove the production of collagenase by cancer cell line and cultured fibroblast of the human skin, and further the collagenolytic activity of several kinds of human tumors including normal human tissues was measured by the culture methods of Gross and Lapiere. The results are following: 1). Cancer cell lines (lung cancer, gastric cancer and colon cancer) had the high ability of collagenase production but cultured fibroblast of the human skin had very low. 2). Collagenolytic activity was much higher in malignant tumors than in normal tissues and benign tumors. 3). These collagenolytic activities were derived from mammlian collagenase by disc electrophoretic patterns of reactive products.