PCR-RFLP 法による HLA-DNA-DQ タイピングと腎移植

Two methods of HLA-DNA typing (PCR-RFLP method and PCR-SSO method) were performed on HLA-DQ in living related, living unrelated and cadaveric renal transplants. These two DNA typing methods allowed more accurate and more detailed typing than the conventional typing method. The effect of DNA histocompatibility of DQA 1 and DQB 1, both typed by the PCR-RFLP method, on clinical outcome and surviving graft rate of renal transplant patients was analyzed. There was no correlation found between the number of mismatches between donor and recipient of DQA 1 typing in cadaveric renal transplants, of DQB 1 typing in cadaveric, living unrelated and living related transplants and the clinical outcome nor the surviving graft rate of renal transplant patients. Both the clinical outcome and the surviving graft rate in the group in which DQ 5 mismatch between donor and recipient was positive were statistically poorer than that in the group in which DQ 5 mismatch was negative. This result suggests the existance of DQ 5 mismatch between donor and recipient greatly influences graft survival after renal transplantation.

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