An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function

Li, Yuying; Wang, Qiong; Jia, Huimin; Ishikawa, Kazuya; Kosami, Ken-Ichi; Ueba, Takahiro; Tsujimoto, Atsumi; Yamanaka, Miki; Yabumoto, Yasuyuki; Miki, Daisuke; Sasaki, Eriko; Fukao, Yoichiro; Fujiwara, Masayuki; Kaneko-Kawano, Takako; Tan, Li; Kojima, Chojiro; Wing, Rod A.; Sebastian, Alfino; Nishimura, Hideki; Fukada, Fumi; Niu, Qingfeng; Shimizu, Motoki; Yoshida, Kentaro; Terauchi, Ryohei; Shimamoto, Ko; Kawano, Yoji

doi:10.1038/s41467-024-48943-5

Permalink : https://ousar.lib.okayama-u.ac.jp/67222

ID	67222
フルテキストURL	fulltext.pdf 2.47 MB
著者	Li, Yuying Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Wang, Qiong Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences Jia, Huimin College of Agronomy, Jiangxi Agricultural University Ishikawa, Kazuya Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences Kosami, Ken-Ichi Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences Ueba, Takahiro Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology Tsujimoto, Atsumi Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology Yamanaka, Miki Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology Yabumoto, Yasuyuki Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology Miki, Daisuke Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences Sasaki, Eriko Faculty of Science, Kyushu University Fukao, Yoichiro Department of Bioinformatics, Ritsumeikan University Fujiwara, Masayuki YANMAR HOLDINGS Co., Ltd. Kaneko-Kawano, Takako College of Pharmaceutical Sciences, Ritsumeikan University Tan, Li Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences Kojima, Chojiro Graduate School of Engineering Science, Yokohama National University Wing, Rod A. Arizona Genomics Institute, School of Plant Sciences, University of Arizona Sebastian, Alfino Institute of Plant Science and Resources, Okayama University Nishimura, Hideki Institute of Plant Science and Resources, Okayama University Fukada, Fumi Institute of Plant Science and Resources, Okayama University Niu, Qingfeng Advanced Academy, Anhui Agricultural University, Research Centre for Biological Breeding Technology Shimizu, Motoki Iwate Biotechnology Research Center Yoshida, Kentaro Graduate School of Agriculture, Kyoto University Terauchi, Ryohei Iwate Biotechnology Research Center Shimamoto, Ko Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology Kawano, Yoji Institute of Plant Science and Resources, Okayama University
抄録	NLR family proteins act as intracellular receptors. Gene duplication amplifies the number of NLR genes, and subsequent mutations occasionally provide modifications to the second gene that benefits immunity. However, evolutionary processes after gene duplication and functional relationships between duplicated NLRs remain largely unclear. Here, we report that the rice NLR protein Pit1 is associated with its paralogue Pit2. The two are required for the resistance to rice blast fungus but have different functions: Pit1 induces cell death, while Pit2 competitively suppresses Pit1-mediated cell death. During evolution, the suppression of Pit1 by Pit2 was probably generated through positive selection on two fate-determining residues in the NB-ARC domain of Pit2, which account for functional differences between Pit1 and Pit2. Consequently, Pit2 lost its plasma membrane localization but acquired a new function to interfere with Pit1 in the cytosol. These findings illuminate the evolutionary trajectory of tandemly duplicated NLR genes after gene duplication.
備考	The version of record of this article, first published in Nature Communications, is available online at Publisher’s website: http://dx.doi.org/10.1038/s41467-024-48943-5
発行日	2024-05-30
出版物タイトル	Nature Communications
巻	15巻
号	1号
出版者	Nature Portfolio
開始ページ	4610
ISSN	2041-1723
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© The Author(s) 2024
論文のバージョン	publisher
PubMed ID	38816417
DOI	10.1038/s41467-024-48943-5
Web of Science KeyUT	001236316700012
関連URL	isVersionOf https://doi.org/10.1038/s41467-024-48943-5
ライセンス	http://creativecommons.org/licenses/by/4.0/
Citation	Li, Y., Wang, Q., Jia, H. et al. An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function. Nat Commun 15, 4610 (2024). https://doi.org/10.1038/s41467-024-48943-5
助成機関名	Chinese Academy of Sciences Shanghai Institutes for Biological Sciences Shanghai Center for Plant Stress Biology CAS Center of Excellence for Molecular Plant Sciences National Natural Science Foundation of China Japan Society for the Promotion of Science Ohara foundation Yakumo Foundation for Environmental Science Ryobi Teien Memory Foundation NOVARTIS Foundation (Japan) for the Promotion of Science Naito foundation Joint Usage/Research Center, the Institute of Plant Science and Resources
助成番号	XDB27040202 173176001000162114 31572073 31772246 20H02988 21H05035 23H02213 23K18030 JPJSBP20237408 2019PB0056 31901985