検索結果 5666 件
| タイトル（別表記） | Habenaria Mosaic Virus, A New Member of Potyvirus from Habenaria radiata Orchid |
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| フルテキストURL | 005_002_155_168.pdf |
| 著者 | 井上 成信| 前田 孚憲| 光畑 興二| Gara I Wayan| |
| 抄録 | A potyvirus, isolated from Habenaria radiaata showing severe mosaic in the leaves named habenaria mosaic virus(HaMV). HaMV was transmitted by inoculation of sap to 6 of 41 species from 4 of 12 families. Systemic infection was cauded in Habenaria radiata, and local infection was produced in Chenopodium amaranticolor, C. quinoa, Spinacia olracea, Tetragonia expansa and Gomphrena globosa. HaMV was also transmitted by Myzus persicae in a non persistent manner, but not by Aphis gossypii. The virus has filamentous particles of c. 750×13nm. Sap from inoculated C. quinoa was infective after dilution to 10-5 but not 10-6, after 10 min at 60℃ but 65℃, or after 8-16 days at 20℃.In ultrathin sections of diseased leaves of Habenaria radiata and inoculated C. quinoa, virus particles were found to disperse in the cytoplasm. Cylindrical inclusions were present in the cytoplasm of infected cells, as pinwheels, scrolls or tubes. The inclusions observed in negatively stained crude sap preparations exhibited fine linear striation at about 5.2 nm intervals.The antiserum to HaMV produced by immunizing a rabbit had a titer of 1/512(precipitin test). HaMV showed distant serological relationships to potyvirus;alstroemeria mosaic virus, bean yellow mosaic virus, calanthe mild mosaic virus, clover yellow vein virus, dendrobium mosaic virus, freesia mosaic virus, turnip mosaic virus and watermelon mosaic virus 2. |
| キーワード | Habenaria orchid Habenaria mosaic potyvirus Habenaria mosaic disease |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 155 |
| 終了ページ | 168 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現 |
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| フルテキストURL | 005_002_145_153.pdf |
| 著者 | 杉本 学| 河合 富佐子| |
| 抄録 | A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one. |
| キーワード | Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 145 |
| 終了ページ | 153 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 |
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| フルテキストURL | 005_002_135_144.pdf |
| 著者 | 今野 晴義| 積木 久明| 山崎 良樹| |
| 抄録 | Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4～0.6% of the starch-free cell walls. |
| キーワード | Cell wall Oryza sativa Pectic polymer |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 135 |
| 終了ページ | 144 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Detection of the Viruses Occurring in Oriental Cymbidium in Japan |
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| フルテキストURL | 004_002_149_162.pdf |
| 著者 | 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A survey of virus diseases occurring in Oriental Cymbidium collected from a commerical nursery and home garden in Japan was conducted in 1991-1994. Identification of the vurus was based on partcle morphology, symptomatology in indicator plants, ultrastructure of infected cells and serology. Four viruses, odontoglossum ringspot tabamovirus(ORSV), cymbidium mosaic potexvirus(CyMV), orchid fleck virus (ORV) and a previously underscribed spherical virus, were found in 27 out of 37 Cymbidium plants tested. ORSV was detected from 11 plants belinging to Cym. ensifolium, Cym. forrestii, Cym. goeringii, Cym. kanran, Cym. sinense and Cymbidium spp. showing chlorotic streaks and/or mild mosaic. CyMV was isolated from only one plant of Cymbidium sp. showing mosaic and necrotic spots on leaves. In negatibvely stained dip preparations from plants infected with ORSV and CyMV, rod shaped particles of ca. 310 nm and flexuous rod-shaped ca. 475 nm in length were observed, respectively. The viruses were reacted strongly with respective antiserum to each virus in immunosorbent electron microcopy and inderect ELISA. OFV was isolated from four plants of Cym. formosanum, Cym. kanran, Cym. sinense and Cymbidium sp. showing mosaic and necrotic flecks. The virus had non-enveloped, bullet-shaped particles about 40×120～150 nm in dip preparation. The undescribed spherical virus, ca. 28 nm diameter, was isolated from 11 plants of Cym. forrestii, Cym. goeringii and Cymbidium spp. showing stunting and chlorotic streaks on newly developed leaves. The virus was mechanically transmitted only to Cymbidium orchids. Previously, we designated it as cymbidium chlorotic mosaic sobemovirus(CyCMV)(Kondo et al,1994),as the virus was considered to be a new member of the genus Sobemovirsu. |
| キーワード | Oriental Cymbidium Odontoglossum ringspot tobamovirus Cymbidium mosaic potexvirus Orchid fleck virus Cymbidium chlorotic mosaic sobemovirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 149 |
| 終了ページ | 162 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313470 |
| タイトル（別表記） | Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan |
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| フルテキストURL | 003_002_163_174.pdf |
| 著者 | 松本 純一| 前田 孚憲| 井上 成信| |
| 抄録 | A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. |
| キーワード | Odontoglossum ringspot virus Calanthe sp. Identification |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 2号 |
| 開始ページ | 163 |
| 終了ページ | 174 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | タバコ懸濁培養細胞におけるアルミニウムの二価鉄、銅およびカドミウム毒性に対する影響 |
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| フルテキストURL | 002_002_181_190.pdf |
| 著者 | 山本 洋子| 張 藝潔| 小野 寛治| 松本 英明| |
| 抄録 | The effects of aluminum (Al) on the cytotoxicity of ferrous iron (Fe(Ⅱ)), copper(Cu) and cadmium(Cd) were studied. Log-phase cells were treated with either FeSO4,CuSO4, or CdCl2 in the presence or absence of AlCl3(120μM) for 18h at pH 4.0. After the treatment, the viability was determined as relative growth of the metal-treated cells to the untreated control cells during the post-treated culture. A single treated with either Al, Fe(Ⅱ) or Cd did not inhibit the growth at the metal concerntrations up to 300 μM, 200 μM and 500 μM, respectively, whereas the growth was markedly inhibited at 15 μM Cu. Thus,the cells were relatively insensitive to Al, Fe(Ⅱ) and Cd and sensitive to Cu. When cells were treated with both Fe(Ⅱ)(120 μM)and Al(120μM), the growth was significantly inhibited and the cellular contents of both Al and Fe increased synergistically. After the treatment with Cu(0 to 10 μM) with or without Al, the cells grew more vigorously when they were treated in the presence of Al, althrouh the Cu content of the cells were not alterd by Al. The presence of Al during the treatmemt with Cd(0 to 2 μM) had no effect on the degree of growth inhibition by Cd. Thus, Al interacts with the toxicity of Fe(Ⅱ), Cu and Cd in different manners; synergistic with Fe(Ⅱ), antagonistic with Cu and apparently no effeco on Cd. |
| キーワード | Aluminum Antagonistic Nicotiana tabacum Suspension culture Synergistic |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 2号 |
| 開始ページ | 181 |
| 終了ページ | 190 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 |
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| フルテキストURL | 001_002_159_166.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. |
| キーワード | Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 159 |
| 終了ページ | 166 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ニンジン懸濁培養細胞の培養液に分泌される細胞外多糖成分 |
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| フルテキストURL | 001_002_091_103.pdf |
| 著者 | 今野 晴義| 山崎 良樹| 加藤 研治| |
| 抄録 | The extracellular polysaccharides have been fractionated from the culture medium of carrot(Daucus carota)cell culrures by precipitation with ethanol and by chromatography on DEAE-Sepharose CL-6B DEAE-Trisacryl M ion-exchange and Bio-Gel A-1.5m gel-permeation.The sugar composition and molecular mass of purified neutral and acidic polymers were determined. The neutral and acidic polymers were treated with purified endo-Β-glucanase from Trichoderma viride and pectic depolymerases,such as endo-pectate lyase from Erwinia carotovora Er. and endo-polygalacturonase from Kluyveromyces fragilis, respectively. The "hairly"(ramified)regions of acidic polymer were sequentially treated with purified α-L-arabinofuranosidase and β-galactosidase from carrot cell cultures, and were further hydrolyzed with 50mM trifluoroacetic for 1 hr at 100℃. From these results, the extracellular polysaccharides secreted from carrot cell cultures are charactarized. |
| キーワード | Carrot Cell suspension culture Cell wall Extracellular polysaccharides Pectic polymer |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 91 |
| 終了ページ | 103 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| 著者 | 村田 芳行| |
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| 発行日 | 1995-02-01 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 84巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 安井 公一| |
|---|---|
| 発行日 | 1995-02-01 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 84巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 松原 幸子| 猪 雅人| 村上 賢治| 鎌田 真由美| 石原 泉| |
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| 発行日 | 1996-02-01 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 85巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 湯原 正高| |
|---|---|
| 発行日 | 1995-02-01 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 84巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 中村 怜之輔| |
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| 発行日 | 1998-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 87巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 高木 茂明| |
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| 発行日 | 1998-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 87巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 寧 波| 久保 康隆| 稲葉 昭次| 中村 怜之輔| |
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| 発行日 | 1997-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 86巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 朝岡 克拓| 今井 俊治| 岡本 五郎| 平野 健| |
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| 発行日 | 1997-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 86巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 後藤 丹十郎| 景山 詳弘| 小西 国義| |
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| 発行日 | 1997-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 86巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 後藤 丹十郎| 正岡 啓史| 景山 詳弘| 小西 国義| |
|---|---|
| 発行日 | 1998-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 87巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 河原 智治| 豊田 和弘| 木場 章範| 一瀬 勇規| 山田 哲治| 白石 友紀| |
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| 発行日 | 1997-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 86巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 古川 裕考| 杉尾 剛| 長澤 透| |
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| 発行日 | 1997-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 86巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |