Hpinz小体に関する研究 第1編 Heinz小体の染色性よりみたその本態について検討

Investigating the mechanism of Heinz's Bodies' staining attitudes for the purpose of clearifiyng the etiology of Heinz's Body, the following results were obtained. 1. Heinz's Bodies were produced in test tubes after adding hydrochloric phenylydrazin to rabbit erythrocytes. In the supravital staining the Heinz's Bodies with twenty -one basic dyestuffs, not only the basic dyes reported by Friedstein and Webster but marked stainings with crystal violette, victorian blue and methyl green were observed. 2. Observing conditions of these Heinz's Bodies, after the fixations with such as methanol, formalin-gas, action, Carony's solution or flames, Triamino Compounds in Triarylmethan, dyes were relatively well stained, the stainings in general were not so good. However, the staining with acid dyestuffs were ex-cellent especially after the fixation with aceton and Carony's solution. 3. On adding the saline solutions containing sodium salicylate, sodium stearate or urea to the rabbit erythrocytes suspension, fein speckled granules smaller than those with phenylhydrazin, well stained by methyl violett, were observed in the peripheral areas of the erythrocytes, in proportion to the Mol concentration of these chemicals. The staininga of Heinz's Body and so were the stainings after the fixations, The granules grew up slowly until maximum size, when no more growth was observed. The optimum concentrations for the growth of the grnules were go to 100 mol urea, 1mol of Sodium stearate or 1 mol of sodium salicylate. 4. In producing Heinz's Bodies no significant difference was noted between the two methods, adding urea after phenyihydrazin to rabbit erythrocyte and the sole administration of phenylhydrazin. 5. The granules, were produced by adding urea to stroma an well as phesylhydrazin, and exhibited aproximate the same staining with Heinz's Body although they were not essentially the same. 6. The granules, corresponding with Heinz's Body, were noted after adding phenylhydrazin to hemoglobin. On adding urea no more granules were produced, but the staining have to changed as they were well stained with trypan blue but not with methyl violett.7. Choleglobin B showed the similar staining to Heinz's Body and changed its staining with urea. No changes were seen in Choleglobin.8. In conclusion, Heinz's Bodies were stemmed from globin of hemoglobin. The theoory that Heinz's Bodies.were resulted from degeneation of stroma was not agreeable, and presumably it was responsible for observation of the substances sinilar to Heinz's Body but essntially different.