On the Difference of the CO2-output of the Frog's Tissue between Different Sex.

The results obtained in this investigation may be summarized as follows : 1. The CO2-output of the male muscle and other tissues is greater than that of the female. 2. The female muscle contains larger amount of water than the female muscle. 3. The muscle immersed 1/2 Ringer solution (or 1/2.5 R.) gave out smaller amount of CO2 per minute than the muscle in 2-Ringer's solution (or 2.5 R.). In spite of the difference in the water content of tissue between different sex, the salt content of the tissue liquid seems to be the same. In other words, larger the water content means larger content of tissue liquid in the tissue. Artificial introduction of water in the tissue or reduction of water content by immersing the tissue in 1/2 or 2-Ringer's solution is quite different from the natural condition occurring between different sex. However both of these conditions influence the gaseous metabolism in the same manner. On an assumption that the gas diffusion in liquid is proportional to the solubility of that gas, the above mentioned difference of CO2-output should be just reversed. Therefore it is not possible to interpret how the water content influences the gaseous metabolism. It may only be stated that the muscle which has a small amount of water to an extent which does not abolish excitability, gives out much CO2 and vice versa.