H-2移植抗原差に基づいて出現する感作リンパ球様細胞の可溶性因子 第1編組織培養法とdiffusion chamberとによる可溶性因子の検索

For the in vitro studying transplantation immunity of mice the soluble factors of sensitized lymphoid cells were investigated by the tissue culture and diffusion chamber methods. As the result it was found that sensitized lymphoid cells obtained from A(H-2(a)) mouse sensitized 9 days previously by a skin graft from C3H (H-2(k)) mouse, and enclosed within the diffusion chamber did not inhibit the proliferation of the primary culture kidney cells from C3H mouse. However, sensitized lymphoid cells obtained from C3H mouse sensitized 9 days previously by a skin graft from A mouse, and enclosed within the diffusion chamber did inhibit the proliferation of the primary culture kidney cells from A mouse. In other words, the soluble factors elaborated by the sensitized lymphoid cells exert cytotoxic action on target cells, and such action seems to grow more marked as the difference in H-2 histocompatibility antigens becomes greater.