ID | 61018 |
フルテキストURL | |
著者 |
Kintaka, Reiko
Donnelly Center for Cellular and Biomolecular Research, Department of Medical Genetics, University of Toronto
Makanae, Koji
Research Core for Interdisciplinary Sciences, Okayama University
Namba, Shotaro
Matching Program Course, Okayama University
Kato, Hisaaki
School of Environmental and Life Science, Okayama University
Kito, Keiji
Department of Life Sciences, School of Agriculture, Meiji University
Ohnuki, Shinsuke
Graduate School of Frontier Sciences, University of Tokyo
Ohya, Yoshikazu
Graduate School of Frontier Sciences, University of Tokyo
Andrews, Brenda J.
Donnelly Center for Cellular and Biomolecular Research, Department of Medical Genetics, University of Toronto
Boone, Charles
Donnelly Center for Cellular and Biomolecular Research, Department of Medical Genetics, University of Toronto
Moriya, Hisao
Research Core for Interdisciplinary Sciences, Okayama University
ORCID
Kaken ID
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抄録 | Overproduction (op) of proteins triggers cellular defects. One of the consequences of overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells under a protein burden is not well characterized. We performed genetic profiling of protein burden by systematic analysis of genetic interactions between GFP-op, surveying both deletion and temperature-sensitive mutants in budding yeast. We also performed genetic profiling in cells with overproduction of triple-GFP (tGFP), and the nuclear export signal-containing tGFP (NES-tGFP). The mutants specifically interacted with GFP-op were suggestive of unexpected connections between actin-related processes like polarization and the protein burden, which was supported by morphological analysis. The tGFP-op interactions suggested that this protein probe overloads the proteasome, whereas those that interacted with NES-tGFP involved genes encoding components of the nuclear export process, providing a resource for further analysis of the protein burden and nuclear export overload.
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発行日 | 2020-11-04
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出版物タイトル |
eLife
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巻 | 9巻
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出版者 | eLife Sciences Publications
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開始ページ | e54080
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ISSN | 2050-084X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © Kintaka et al.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.7554/eLife.54080
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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助成機関名 |
日本学術振興会
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助成番号 | 17H03618
15KK0258
18K19300
20H03242
19H03205
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