ID | 47727 |
フルテキストURL | |
著者 |
Ohtsuki, Takashi
Department of Biology, Faculty of Science, Okayama University
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Otsuki, Mariko
Department of Biology, Faculty of Science, Okayama University
Murakami, Yousuke
Department of Biology, Faculty of Science, Okayama University
Maekawa, Tetsuya
Department of Biology, Faculty of Science, Okayama University
Yamamoto, Takashi
Department of Mathematical and Life Science, Graduate School of Science, Hiroshima University
Akasaka, Koji
Misaki Marine Biological Station, University of Tokyo
Takeuchi, Sakae
Department of Biology, Faculty of Science, Okayama University
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Takahashi, Sumio
Department of Biology, Faculty of Science, Okayama University
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抄録 | Insulin-like growth factor-I (IGF-I) gene generates several IGF-I mRNA variants by alternative splicing. Two promoters are present in mouse IGF-I gene. Each promoter encodes two IGF-I mRNA variants (IGF-IA and IGF-IB mRNAs). Variants differ by the presence (IGF-IB) or absence (IGF-IA) of a 52-bp insert in the E domain-coding region. Functional differences among IGF-I mRNAs, and regulatory mechanisms for alternative splicing of IGF-I mRNA are not yet known. We analyzed the expression of mouse IGF-IA and IGF-IB mRNAs using SYBR Green real-time RT-PCR. In the liver, IGF-I mRNA expression increased from 10 days of age to 45 days. In the uterus and ovary, IGF-I mRNA expression increased from 21 days of age, and then decreased at 45 days. In the kidney, IGF-I mRNA expression decreased from 10 days of age. IGF-IA mRNA levels were higher than IGF-IB mRNA levels in all organs examined. Estradiol-17 beta (E2) treatment in ovariectomized mice increased uterine IGF-IA and IGF-IB mRNA levels from 3 hr after injection, and highest levels for both mRNAs were detected at 6 hr, and relative increase was greater for IGF-IB mRNA than for IGF-IA mRNA. These results suggest that expression of IGF-I mRNA variants is regulated in organ-specific and age-dependent manners, and estrogen is involved in the change of IGF-I mRNA variant expression.
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キーワード | insulin like growth factor-I (IGF-I)
uterus
estradiol
mouse
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発行日 | 2005-09
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出版物タイトル |
Zoological Science
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巻 | 22巻
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号 | 9号
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出版者 | Zoological Society of Japan
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出版者(別表記) | 日本動物学会
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開始ページ | 1011
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終了ページ | 1021
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ISSN | 0289-0003
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NCID | AA10545874
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | © 2005 Zoological Society of Japan
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
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