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ID 47727
フルテキストURL
著者
Ohtsuki, Takashi Department of Biology, Faculty of Science, Okayama University
Otsuki, Mariko Department of Biology, Faculty of Science, Okayama University
Murakami, Yousuke Department of Biology, Faculty of Science, Okayama University
Maekawa, Tetsuya Department of Biology, Faculty of Science, Okayama University
Yamamoto, Takashi Department of Mathematical and Life Science, Graduate School of Science, Hiroshima University
Akasaka, Koji Misaki Marine Biological Station, University of Tokyo
Takeuchi, Sakae Department of Biology, Faculty of Science, Okayama University
Takahashi, Sumio Department of Biology, Faculty of Science, Okayama University
抄録
Insulin-like growth factor-I (IGF-I) gene generates several IGF-I mRNA variants by alternative splicing. Two promoters are present in mouse IGF-I gene. Each promoter encodes two IGF-I mRNA variants (IGF-IA and IGF-IB mRNAs). Variants differ by the presence (IGF-IB) or absence (IGF-IA) of a 52-bp insert in the E domain-coding region. Functional differences among IGF-I mRNAs, and regulatory mechanisms for alternative splicing of IGF-I mRNA are not yet known. We analyzed the expression of mouse IGF-IA and IGF-IB mRNAs using SYBR Green real-time RT-PCR. In the liver, IGF-I mRNA expression increased from 10 days of age to 45 days. In the uterus and ovary, IGF-I mRNA expression increased from 21 days of age, and then decreased at 45 days. In the kidney, IGF-I mRNA expression decreased from 10 days of age. IGF-IA mRNA levels were higher than IGF-IB mRNA levels in all organs examined. Estradiol-17 beta (E2) treatment in ovariectomized mice increased uterine IGF-IA and IGF-IB mRNA levels from 3 hr after injection, and highest levels for both mRNAs were detected at 6 hr, and relative increase was greater for IGF-IB mRNA than for IGF-IA mRNA. These results suggest that expression of IGF-I mRNA variants is regulated in organ-specific and age-dependent manners, and estrogen is involved in the change of IGF-I mRNA variant expression.
キーワード
insulin like growth factor-I (IGF-I)
uterus
estradiol
mouse
発行日
2005-09
出版物タイトル
Zoological Science
22巻
9号
出版者
Zoological Society of Japan
出版者(別表記)
日本動物学会
開始ページ
1011
終了ページ
1021
ISSN
0289-0003
NCID
AA10545874
資料タイプ
学術雑誌論文
言語
English
著作権者
© 2005 Zoological Society of Japan
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT