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ID 47700
フルテキストURL
著者
Shiraga, Masahiro Department of Biology, Faculty of Science, Okayama University
Komatsu, Noriko Department of Biology, Faculty of Science, Okayama University
Teshigawara, Kiyoshi Department of Biology, Faculty of Science, Okayama University
Okada, Akinobu Department of Biology, Faculty of Science, Okayama University
Takeuchi, Sakae Department of Biology, Faculty of Science, Okayama University
Fukamachi, Hiroshi Department of Biological Sciences, Graduate School of Science, University of Tokyo
Takahashi, Sumio Department of Biology, Faculty of Science, Okayama University
抄録
Epidermal growth factor (EGF) is one of growth factors that are thought to mediate the stimulatory effects of estrogen on the proliferation of uterine epithelial cells. The present study was attempted to obtain direct evidence for the mitogenic effects of EGF on uterine epithelial cells, and to prove that EGF and EGF receptors are expressed in these cells. Mouse uterine epithelial cells were isolated from immature female mice and cultured with or without EGF for 5 days. EGF (1 to 100 ng/ml) significantly increased the number of uterine epithelial cells, and the maximal growth (141.9+/-8.3% of controls) was obtained at a dose of 10 ng/ml. In addition, EGF (0.1 to 100 ng/ml) increased the number of DNA-synthesizing cells immunocytochemically detected by bromodeoxyuridine uptake to the nucleus. Northern blot analysis revealed that the uterine epithelial cells expressed both EGF mRNA (4.7 kb) and EGF receptor mRNAs (10.5, 6.6, and 2.7 kb) These results suggest that the proliferation of uterine epithelial cells is regulated by the paracrine and/ or autocrine action of EGF. Our previous study demonstrated the mitogenic effect of IGF-I on uterine epithelial cells. To examine whether the EGF- and IGF-I signaling act at the same level in the regulation of the proliferation of uterine epithelial cells, the cultured cells were simultaneously treated with IGF-I and EGF. IGF-I was found to additively stimulate the mitogenic effects of EGF, suggesting that the EGF-induced growth of uterine epithelial cells is distinct from IGF-l-induced growth.
発行日
2000-07
出版物タイトル
Zoological Science
17巻
5号
出版者
Zoological Society of Japan
出版者(別表記)
日本動物学会
開始ページ
661
終了ページ
666
ISSN
0289-0003
NCID
AA10545874
資料タイプ
学術雑誌論文
言語
English
著作権者
© 2000 Zoological Society of Japan
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT