Kitamatsu, Mizuki Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University
We describe the fluorescence quenching-based quantification of complementary parallel and antiparallel hybrids of DNAs with pyrrolidine-based oxypeptide nucleic acids (POPNAs). When BODIPY-modified DNAs as fluorescent probe formed hybrids with complementary POPNAs, fluorescence of the BODIPY was effectively quenched by the guanine unit and the Lys unit on the POPNAs. The orientations of hybrids of POPNA with DNA were estimated by the quenching efficiencies of two BODIPY-modified DNAs . As a result, we clarified that configurations of POPNAs affect the extent of orientation of the hybrid duplexes.
The Royal Society of Chemistry
© The Royal Society of Chemistry
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