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ID 56096
フルテキストURL
著者
Uemura, Ryota Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Ogura, Mikako Faculty of Agriculture , Okayama University
Matsumaru, Chihiro Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Akiyama, Tsuyoshi Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Maeda, Megumi Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University Kaken ID researchmap
Kimura, Yoshinobu Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University Kaken ID publons researchmap
抄録
Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials
キーワード
Acidic PNGase
FNG: free N-glycan
Fuc: L-fucose
Gal: D-galactose
GlcNAc: N-acetyl-D-glucosamine
HPLC: high-performance liquid chromatography
Man: D-mannose
NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc
NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc
NeuNAc: N-acetylneuraminic acid
PA-: pyridylamino
PNGase-A: aPNGase from almond seed
PNGase-Le: aPNGase from tomato (Solanum lycopersium L.)
PNGase: peptide:N-glycanase
PTC: plant complex type
RCA120: Ricinus communis agglutinin (120 kDa)
RP-HPLC: reversed-phase HPLC
SF-HPLC: size-fractionation HPLC
Xyl: D-xylose
affinity chromatography
enzyme assay
free N-glycans
transgenic plant
発行日
2018-04
出版物タイトル
Bioscience, Biotechnology, and Biochemistry
82巻
7号
出版者
Taylor & Francis
開始ページ
1172
終了ページ
1175
ISSN
09168451
NCID
AA10824164
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1080/09168451.2018.1459464
助成機関名
文部科学省
助成番号
15K07841
15KK0282
17K0819709