ID | 55422 |
フルテキストURL |
fig_all.zip
1.54 MB
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著者 |
Hara, Chikako
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kubota, Satoshi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
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Nishida, Takashi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hiasa, Miki
Department of Membrane Biochemistry , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Hattori, Takako
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Aoyama, Eriko
Advanced Research Center for Oral and Craniofacial Sciences , Okayama University Dental School
ORCID
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Moriyama, Yoshinori
Department of Membrane Biochemistry , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kamioka, Hiroshi
Department of Orthodontics , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Takigawa, Masaharu
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | OBJECTIVES:
Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration.
METHODS:
Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells.
RESULTS:
Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype.
CONCLUSIONS:
Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
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キーワード | CCN family
Cartilage
Megakaryocyte
Platelet
Regeneration
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備考 | This is an Accepted Manuscript of an article published by Taylor & Francis Group
This fulltext availavle in Nov 2017
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発行日 | 2016-11
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出版物タイトル |
Modern Rheumatology
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巻 | 26巻
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号 | 6号
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出版者 | Taylor & Francis
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開始ページ | 940
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終了ページ | 949
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ISSN | 1439-7595
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NCID | AA1157187X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3109/14397595.2016.1155255
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