著者 Uemura, Ryota| Ogura, Mikako| Matsumaru, Chihiro| Akiyama, Tsuyoshi| Maeda, Megumi| Kimura, Yoshinobu|
抄録 Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials
キーワード Acidic PNGase FNG: free N-glycan Fuc: L-fucose Gal: D-galactose GlcNAc: N-acetyl-D-glucosamine HPLC: high-performance liquid chromatography Man: D-mannose NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc NeuNAc: N-acetylneuraminic acid PA-: pyridylamino PNGase-A: aPNGase from almond seed PNGase-Le: aPNGase from tomato (Solanum lycopersium L.) PNGase: peptide:N-glycanase PTC: plant complex type RCA120: Ricinus communis agglutinin (120 kDa) RP-HPLC: reversed-phase HPLC SF-HPLC: size-fractionation HPLC Xyl: D-xylose affinity chromatography enzyme assay free N-glycans transgenic plant
備考 This fulltext will be available in Apr 2019
発行日 2018-04
出版物タイトル Bioscience, Biotechnology, and Biochemistry
82巻
7号
出版者 Taylor & Francis
開始ページ 1172
終了ページ 1175
ISSN 09168451
NCID AA10824164
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 29658432
DOI 10.1080/09168451.2018.1459464
関連URL isVersionOf https://doi.org/10.1080/09168451.2018.1459464
フルテキストURL BBB_81_7_1405.pdf
著者 Tanabe, Chinatsu| Kaori, Furuta| Megumi, Maeda| Yoshinobu, Kimura|
抄録 An effective method to prepare plant complex type (PCT) N-glycans in large amounts has been required to evaluate their immunological activity. In this study, we found that glycoproteins in bamboo shoots predominantly carry PCT N-glycans including the Lewis a epitope-containing ones, suggesting that bamboo shoot is an excellent source for the plant antigenic glycans to synthesize immunoactive neoglycopolymers.
キーワード Phyllostachys edulis antigenic N-glycans bamboo shoot plant N-glycans plant glycoproteins
発行日 2017-05
出版物タイトル Bioscience, Biotechnology, and Biochemistry
81巻
7号
出版者 Taylor & Francis
開始ページ 1405
終了ページ 1408
ISSN 0916-8451
NCID AA10824164
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 28471334
DOI 10.1080/09168451.2017.1320519
Web of Sience KeyUT 000402992500021
関連URL isVersionOf https://doi.org/10.1080/09168451.2017.1320519
フルテキストURL Mod_Rheumatol_26_6_940.pdf fig.pdf tbl.jpg fig_all.zip
著者 Hara, Chikako| Kubota, Satoshi| Nishida, Takashi| Hiasa, Miki| Hattori, Takako| Aoyama, Eriko| Moriyama, Yoshinori| Kamioka, Hiroshi| Takigawa, Masaharu|
抄録 OBJECTIVES: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. METHODS: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. RESULTS: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. CONCLUSIONS: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
キーワード CCN family Cartilage Megakaryocyte Platelet Regeneration
備考 This is an Accepted Manuscript of an article published by Taylor & Francis Group This fulltext availavle in Nov 2017
発行日 2016-11
出版物タイトル Modern Rheumatology
26巻
6号
出版者 Taylor & Francis
開始ページ 940
終了ページ 949
ISSN 1439-7595
NCID AA1157187X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 26915735
DOI 10.3109/14397595.2016.1155255
Web of Sience KeyUT 000393121500022
関連URL isVersionOf https://doi.org/10.3109/14397595.2016.1155255
フルテキストURL Mod_Rheumatol_26_5_730.pdf
著者 Sada, Ken-ei| Harigai, Masayoshi| Amano, Koichi| Atsumi, Tatsuya| Fujimoto, Shouichi| Yuzawa, Yukio| Takasaki, Yoshinari| Banno, Shogo| Sugihara, Takahiko| Kobayashi, Masaki| Usui, Joichi| Yamagata, Kunihiro| Homma, Sakae| Dobashi, Hiroaki| Tsuboi, Naotake| Ishizu, Akihiro| Sugiyama, Hitoshi| Okada, Yasunori| Arimura, Yoshihiro| Matsuo, Seiichi| Makino, Hirofumi|
抄録 OBJECTIVE: To compare disease severity classification systems for six-month outcome prediction in patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). METHODS: Patients with newly diagnosed AAV from 53 tertiary institutions were enrolled. Six-month remission, overall survival, and end-stage renal disease (ESRD)-free survival were evaluated. RESULTS: According to the European Vasculitis Study Group (EUVAS)-defined disease severity, the 321 enrolled patients were classified as follows: 14, localized; 71, early systemic; 170, generalized; and 66, severe disease. According to the rapidly progressive glomerulonephritis (RPGN) clinical grading system, the patients were divided as follows: 60, grade I; 178, grade II; 66, grade III; and 12, grade IV. According to the Five-Factor Score (FFS) 2009, 103, 109, and 109 patients had ≤1, 2, and ≥3 points, respectively. No significant difference in remission rates was found in any severity classification. The overall and ESRD-free survival rates significantly differed between grades I/II, III, and IV, regardless of renal involvement. Severe disease was a good predictor of six-month overall and ESRD-free survival. The FFS 2009 was useful to predict six-month ESRD-free survival but not overall survival. CONCLUSIONS: The RPGN grading system was more useful to predict six-month overall and ESRD-free survival than the EUVAS-defined severity or FFS 2009.
キーワード Antineutrophil cytoplasmic antibody-associated vasculitis Eosinophilic granulomatosis with polyangiitis Granulomatosis with polyangiitis Inception cohort Microscopic polyangiitis
備考 This is an Accepted Manuscript of an article published by Taylor & Francis Group
発行日 2016-03-11
出版物タイトル Modern Rheumatology
26巻
5号
出版者 Taylor & Francis
開始ページ 730
終了ページ 737
ISSN 1439-7595
NCID AA1157187X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 26873424
DOI 10.3109/14397595.2016.1140274
Web of Sience KeyUT 000393120600018
関連URL https://doi.org/10.3109/14397595.2016.1140274
フルテキストURL Commun_Algebra_45_4_1509.pdf
著者 Hashimoto, Mitsuyasu|
抄録 We prove a version of Grothendieck’s descent theorem on an ‘enriched’ principal fiber bundle, a principal fiber bundle with an action of a larger group scheme. Using this, we prove the isomorphisms of the equivariant Picard and the class groups arising from such a principal fiber bundle.
キーワード Class group descent theory Picard group principal fiber bundle
備考 This is an Accepted Manuscript of an article published by Taylor & Francis Group. Fulltext available in Oct. 2017
発行日 2016-10-07
出版物タイトル Communications in Algebra
45巻
4号
出版者 Taylor & Francis
開始ページ 1509
終了ページ 1532
ISSN 0092-7872
NCID AA00611371
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
DOI 10.1080/00927872.2016.1178270
Web of Sience KeyUT 000389500800010
関連URL isVersionOf https://doi.org/10.1080/00927872.2016.1178270
フルテキストURL Acta_Otolaryngol_137_5_529.pdf Acta_Otolaryngol_137_5_529_image.pdf
著者 Kariya, Shin| Okano, Mitsuhiro| Oto, Takahiro| Higaki, Takaya| Haruna, Takenori| Noda, Yohei| Nishizaki, Kazunori|
抄録 CONCLUSIONS: Chronic rhinosinusitis diagnosed according to the European Position Paper on Rhinosinusitis and Nasal Polyps 2012, not by computed tomography alone, is one of the prognostic factors affecting long-term survival in patients with lung transplantation. Endoscopic sinus surgery might play a beneficial role in the management of lung transplantation recipients with chronic rhinosinusitis. OBJECTIVE: To show the effect of paranasal sinus infection on post-lung transplantation survival. METHOD: Lung transplantation recipients were included in this study. Computed tomography was performed before and after lung transplantation. The severity of chronic rhinosinusitis was evaluated by Lund-Mackay scoring system. The survival rate was calculated by the Kaplan-Meier method. RESULTS: One hundred and forty-eight patients received lung transplantation for various indications. Chronic rhinosinusitis was found in 18.9% (28/148) of the lung transplantation recipients. Of 28 patients with chronic rhinosinusitis, seven patients underwent endoscopic sinus surgery due to persistent post-nasal drip. The recipients with chronic rhinosinusitis who did not receive endoscopic sinus surgery (n = 21) showed a significantly lower survival rate as compared to the patients without chronic rhinosinusitis. There was no statistically significant difference in the survival rate between the recipients with (n = 50) and without (n = 98) paranasal sinus abnormality on computed tomography.
キーワード Lung transplant bronchiolitis obliterans infection; pneumonia survival rate
備考 2018年5月公開予定
発行日 2017-05
出版物タイトル Acta Oto-laryngologica
137巻
5号
出版者 Taylor & Francis
開始ページ 529
終了ページ 533
ISSN 0001-6489
NCID AA0050923X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 27809696
DOI 10.1080/00016489.2016.1247497
Web of Sience KeyUT 000400167100014
関連URL https://doi.org/10.1080/00016489.2016.1247497