ID | 60852 |
フルテキストURL | |
著者 |
Maeda, Takanori
College of Bioresource Sciences, Nihon University
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抄録 | Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions.
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キーワード | Rabies
ORF4 Protein
Sucrose Density Gradient Centrifugation
Coil Coil Structure
Potential Glycosylation Site
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備考 | This is a post-peer-review, pre-copyedit version of an article published in Archives of Virology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00705-008-0268-6.
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発行日 | 2008-12-06
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出版物タイトル |
Archives of Virology
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巻 | 154巻
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号 | 1号
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出版者 | Springer
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開始ページ | 37
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終了ページ | 45
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ISSN | 0304-8608
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NCID | AA00548901
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1007/s00705-008-0268-6
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