ID | 57498 |
フルテキストURL | |
著者 |
Ichinose, Yuki
Graduate School of Environmental and Life ScienceOkayama University
ORCID
Kaken ID
publons
researchmap
Tasaka, Yousuke
Graduate School of Environmental and Life ScienceOkayama University
Yamamoto, Satoru
Graduate School of Environmental and Life ScienceOkayama University
Inoue, Yuko
Faculty of AgricultureOkayama University
Takata, Motohiro
Graduate School of Environmental and Life ScienceOkayama University
Nakatsu, Yukiko
Graduate School of Environmental and Life ScienceOkayama University
Taguchi, Fumiko
Graduate School of Environmental and Life ScienceOkayama University
Yamamoto, Mikihiro
Graduate School of Environmental and Life ScienceOkayama University
Toyoda, Kazuhiro
Graduate School of Environmental and Life ScienceOkayama University
Noutoshi, Yoshiteru
Graduate School of Environmental and Life ScienceOkayama University
Matsui, Hidenori
Graduate School of Environmental and Life ScienceOkayama University
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抄録 | Quorum sensing (QS) is a mechanism for bacterial cell-cell communication using QS signals. N-acyl-homoserine lactones (AHLs), QS signals in Pseudomonas syringae pv. tabaci (Pta) 6605, are synthesized by an AHL synthase (PsyI) and recognized by the cognate transcription factor PsyR. To reveal the role of PsyR in virulence, we generated a psyR mutant and complemented strains of Pta 6605 and found that the psyR mutant is remarkably reduced in AHL production and ability to cause disease and propagate in host tobacco leaves. The phenotypes of complemented strains were restored to that of the wild type (WT). Because the psyR mutant lost nearly all AHL production, we investigated the function of PsyR in the transcription of psyI and production of AHL. Electrophoretic mobility shift assays suggested that the recombinant PsyR protein binds the promoter region of psyI but not psyR without AHL. The addition of AHL did not significantly affect this binding. The binding core sequence of this region was identified as a 20-bp lux box-like sequence. To reveal the function of PsyR and AHL on psyI transcription, we constructed a psyI promoter::lacZYA chimeric reporter gene, and inserted it into the WT and psyI mutant of Pta 6605. beta-galactosidase activity increased in a bacterial density-dependent manner in the WT and also in a psyI mutant after the addition of exogenous AHL. These results indicate that the solo PsyR binds the lux box in the psyI promoter and activates transcription in the concomitant presence of AHL.
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キーワード | AHL
PsyI
PsyR
Quorum sensing
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備考 | This is a post-peer-review, pre-copyedit version of an article published in Journal of General Plant Pathology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10327-019-00893-3.
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発行日 | 2019-11-11
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出版物タイトル |
Journal of General Plant Pathology
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巻 | 86巻
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出版者 | Springer
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開始ページ | 124
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終了ページ | 133
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ISSN | 13452630
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NCID | AA11449093
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | author
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DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1007/s10327-019-00893-3
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Citation | Ichinose, Y., Tasaka, Y., Yamamoto, S. et al. J Gen Plant Pathol (2019). https://doi.org/10.1007/s10327-019-00893-3
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助成機関名 |
文部科学省
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助成番号 | 15H04458
19H02956
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