| ID | 33000 |
| フルテキストURL | |
| 著者 |
Toeda, Kenichi
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Nakamura, Keigo
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Hirohata, Satoshi
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
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Hatipoglu, Omer F.
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry
Demircan, Kadir
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry
Yamawaki, Hitoshi
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Ogawa, Hiroko
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Kusachi, Shozo
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Shiratori, Yasushi
Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Ninomiya, Yoshifumi
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry
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| 抄録 | Versican, a large chondroitin sulfate proteoglycan, plays a role in conditions such as wound healing and tissue remodelling. To test the hypothesis that versican expression is transiently upregulated and plays a role in the infarcted heart, we examined its expression in a rat model of myocardial infarction. Northern blot analysis demonstrated increased expression of versican mRNA. Quantitative real-time RT-PCR analysis revealed that versican mRNA began to increase as early as 6 h and reached its maximal level 2 days after coronary artery ligation. Versican mRNA then gradually decreased, while the mRNA of decorin, another small proteoglycan, increased thereafter. Versican mRNA was localized in monocytes, as indicated by CD68-positive staining, around the infarct tissue. The induction of versican mRNA was accelerated by ischemia/reperfusion (I/R), which was characterized by massive cell infiltration and enhanced inflammatory response. To examine the alteration of versican expression in monocytes/macrophages, we isolated human peripheral blood mononuclear cells and stimulated them with granulocyte/macrophage colony-stimulating factor (GM-CSF). Stimulation of mononuclear cells with GM-CSF increased the expression of versican mRNA as well as cytokine induction. The production of versican by monocytes in the infarct area represents a novel finding of the expression of an extracellular matrix gene by monocytes in the infarcted heart. We suggest that upregulation of versican in the infarcted myocardium may have a role in the inflammatory reaction, which mediates subsequent chemotaxis in the infarcted heart.
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| キーワード | coronary artery disease
cytokine
extracellular matrix
GM-CSF
monocyte
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| 備考 | Digital Object Identifer:10.1007/s11010-005-8051-4
Published with permission from the copyright holder. This is the author's copy, as published in Molecular and Cellular Biochemistry, December 2005, Volume 280, Issue 1-2, Pages 47-56. Publisher URL:http://dx.doi.org/10.11997 The Roya007/s11010-005-8051-4 Direct access to Thomson Web of Science record Copyright © 2005 Springer. All rights reserved. |
| 発行日 | 2005-12
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| 出版物タイトル |
Molecular and Cellular Biochemistry
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| 巻 | 280巻
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| 号 | 1-2号
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| 出版者 | Springer
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| 開始ページ | 47
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| 終了ページ | 56
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| ISSN | 0300-8177
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| NCID | AA00745800
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| 資料タイプ |
学術雑誌論文
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| 言語 |
英語
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| 著作権者 | Springer
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| 論文のバージョン | author
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| 査読 |
有り
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| DOI | |
| PubMed ID | |
| Web of Science KeyUT | |
| Submission Path | biology_general/34
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