A novel model of liver cancer stem cells developed from induced pluripotent stem cells

Afify, Said M.; Calle, Anna Sanchez; Hassan, Ghmkin; Kumon, Kazuki; Nawara, Hend M.; Zahra, Maram H.; Mansour, Hager M.; Khayrani, Apriliana Cahya; Alam, Md Jahangir; Du, Juan; Seno, Akimasa; Iwasaki, Yoshiaki; Seno, Masaharu

doi:10.1038/s41416-020-0792-z

Permalink : http://escholarship.lib.okayama-u.ac.jp/61237

ID	61237
フルテキストURL	fulltext.pdf 6.59 MB
著者	Afify, Said M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University ORCID Calle, Anna Sanchez Division of Molecular and Cellular Medicine, National Cancer Center Research Institute Hassan, Ghmkin Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID publons Kumon, Kazuki Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University Nawara, Hend M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University Zahra, Maram H. Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Mansour, Hager M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University Khayrani, Apriliana Cahya Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University Alam, Md Jahangir Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University Du, Juan Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University Seno, Akimasa Okayama University Research Laboratory of Stem Cell Engineering in Detroit, IBio, Wayne State University ORCID Kaken ID publons researchmap Iwasaki, Yoshiaki Department of Gastroenterology and Hepatology, Graduate School of Medicine, Okayama University ORCID Kaken ID publons researchmap Seno, Masaharu Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
抄録	Background Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown. Methods In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed. Results The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers. Conclusions We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.
キーワード	Cancer models Cancer stem cells
発行日	2020-03-17
出版物タイトル	British Journal of Cancer
巻	122巻
号	9号
出版者	Springer Nature
開始ページ	1378
終了ページ	1390
ISSN	0007-0920
NCID	AA00574355
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© Authors
論文のバージョン	publisher
PubMed ID	32203212
DOI	10.1038/s41416-020-0792-z
Web of Science KeyUT	000519841900001
関連URL	isVersionOf https://doi.org/10.1038/s41416-020-0792-z
ライセンス	http://creativecommons. org/licenses/by/4.0/