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ID 48980
フルテキストURL
著者
Matsuura, Koji Research Core for Interdisciplinary Sciences, Okayama University Kaken ID publons researchmap
Kuroda, Yuka Research Core for Interdisciplinary Sciences, Okayama University
Yamashita, Keisuke Department of Animal Science, Faculty of Agriculture, Okayama University
Funahashi, Hiroaki Department of Animal Science, Faculty of Agriculture, Okayama University ORCID Kaken ID publons researchmap
抄録
Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.
キーワード
Adsorption
Porcine sperm motility
Silicone elastomer
Trajectories
発行日
2010-10-12
出版物タイトル
Journal of Reproduction and Development
57巻
1号
出版者
Society for Reproduction and Development
開始ページ
163
終了ページ
167
ISSN
0916-8818
資料タイプ
学術雑誌論文
プロジェクト
異分野融合先端研究コア
オフィシャル URL
https://www.jstage.jst.go.jp/article/jrd/57/1/57_10-075N/_article
言語
English
著作権者
Copyright © 2011 Society for Reproduction and Development
論文のバージョン
author
査読
有り
DOI
PubMed ID
Web of Science KeyUT