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ID 48975
フルテキストURL
著者
Koike, Takayuki Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University
Matsuura, Koji Research Core for Interdisciplinary Sciences, Okayama University Kaken ID publons researchmap
Naruse, Keiji Department of Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Funahashi, Hiroaki Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
抄録
Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P<0.05). These observations indicate that tilting culture during IVM and IVC in chemically defined media improves the quality of blastocyst, as determined by the number of cells per blastocyst, without any effects on penetrability and developmental competence.
キーワード
Inclining device
In vitro culture
In vitro fertilization
Oocytes
Pig
発行日
2010-10
出版物タイトル
Journal of Reproduction and Development
56巻
5号
出版者
Society for Reproduction and Development
開始ページ
552
終了ページ
557
ISSN
0916-8818
資料タイプ
学術雑誌論文
プロジェクト
異分野融合先端研究コア
オフィシャル URL
https://www.jstage.jst.go.jp/article/jrd/56/5/56_10-041H/_article
言語
English
著作権者
Copyright © 2010 Society for Reproduction and Development
論文のバージョン
author
査読
有り
DOI
PubMed ID
Web of Science KeyUT