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ID 48121
フルテキストURL
著者
Matsuura, Eiji Department of Cell Chemistry, Institute of Molecular and Cellular Biology, Okayama University School of Medicine
Igarashi, Yoshiko Immunology Laboratory, Diagnostics Division, Yamasa Corporation
Yasuda, Tatsuji Department of Cell Chemistry, Institute of Molecular and Cellular Biology, Okayama University School of Medicine
Triplett, Douglas A. Department of Pathology, Ball Memorial Hospital
Koike, Takao Department of Medicine II, Hokkaido University School of Medicine
抄録
Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta(2)-glycoprotein I (beta(2)-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C=O moieties. aCL derived from either APS patients or (NZW x BXSB)F-1 mice bound to beta(2)-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to beta(2)-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with beta(2)-GPI but were unaffected by addition of excess beta(2)-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-beta(2)-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the beta(2)-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on beta(2)-GPI. This epitope is expressed by a conformational change occurring when beta(2)-GPI interacts with an oxygen-substituted solid phase surface.
発行日
1994-02-01
出版物タイトル
Journal of Experimental Medicine
179巻
2号
出版者
Rockefeller University Press
開始ページ
457
終了ページ
462
ISSN
0022-1007
NCID
AA00697559
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/3929
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
© 1994 Rockefeller University Press
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT