ID | 67525 |
フルテキストURL | |
著者 |
Kawase, Tomoka
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Debnath, Anusuya
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mizuno, Tamaki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
researchmap
Miyake, Yui
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
|
抄録 | Vibrio vulnificus is a Gram-negative estuarine bacterium that causes infection in immuno-compromised patients, eels, and shrimp. V. vulnificus NCIMB2137, a metalloprotease-negative strain isolated from a diseased eel, produces a 45-kDa chymotrypsin-like alkaline serine protease known as VvsA. The gene encoding vvsA also includes another gene, vvsB with an unknown function; however, it is assumed to be an essential molecular chaperone for the maturation of VvsA. In the present study, we used an in vitro cell-free translation system to examine the maturation pathway of VvsA. We individually expressed the vvsA and vvsB genes and detected their mRNAs. However, the sample produced from vvsA did not exhibit protease activity. A sodium dodecyl sulfate (SDS) analysis detected the VvsB protein, but not the VvsA protein. A Western blotting analysis using a histidine (His)-tag at the amino terminus of proteins also showed no protein production by vvsA. These results suggested the translation, but not the transcription of vvsA. Factors derived from Escherichia coli were used in the in vitro cell-free translation system employed in the present study. The operon of the serine protease gene containing vvsA and vvsB was expressed in E. coli. Although serine proteases were produced, they were cleaved at different sites and no active mature forms were detected. These results indicate that the operon encoding vvsA and vvsB is a gene constructed to be specifically expressed in V. vulnificus.
|
キーワード | Vibrio vulnificus serine protease
intermolecular chaperone
cell-free translation system
|
発行日 | 2022-11-01
|
出版物タイトル |
Biological and Pharmaceutical Bulletin
|
巻 | 45巻
|
号 | 11号
|
出版者 | Pharmaceutical Society of Japan
|
開始ページ | 1596
|
終了ページ | 1601
|
ISSN | 0918-6158
|
NCID | AA10885497
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
OAI-PMH Set |
岡山大学
|
著作権者 | © 2022 The Pharmaceutical Society of Japan
|
論文のバージョン | publisher
|
PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1248/bpb.b22-00106
|
助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
|