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Sasai, Rumi Graduate School of Environmental and Life Science, Okayama University
Tabuchi, Hiroaki Kyusyu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization
Shirasawa, Kenta Kazusa DNA Research Institute
Kishimoto, Kazuki Graduate School of Environmental and Life Science, Okayama University
Sato, Shusei Graduate School of Life Science, Tohoku University
Okada, Yoshihiro Kyusyu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization
Kuramoto, Akihide Graduate School of Agriculture, Kyoto University
Kobayashi, Akira Kyusyu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization
Isobe, Sachiko Kazusa DNA Research Institute
Tahara, Makoto Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
The southern root-knot nematode, Meloidogyne incognita, is a pest that decreases yield and the quality of sweetpotato [Ipomoea batatas (L.) Lam.]. There is a demand to produce resistant cultivars and develop DNA markers to select this trait. However, sweetpotato is hexaploid, highly heterozygous, and has an enormous genome (similar to 3 Gb), which makes genetic linkage analysis difficult. In this study, a high-density linkage map was constructed based on retrotransposon insertion polymorphism, simple sequence repeat, and single nucleotide polymorphism markers. The markers were developed using F-1 progeny between J-Red, which exhibits resistance to multiple races of M. incognita, and Choshu, which is susceptible to multiple races of such pest. Quantitative trait locus (QTL) analysis and a genome-wide association study detected highly effective QTLs for resistance against three races, namely, SP1, SP4, and SP6-1, in the Ib01-6 J-Red linkage group. A polymerase chain reaction marker that can identify genotypes based on single nucleotide polymorphisms located in this QTL region can discriminate resistance from susceptibility in the F-1 progeny at a rate of 70%. Thus, this marker could be helpful in selecting sweetpotato cultivars that are resistant to multiple races of M. incognita.
Oxford University Press
© The Author(s) 2019.
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