このエントリーをはてなブックマークに追加
ID 53139
フルテキストURL
著者
Onoda, Akihisa Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Hosoya, Osamu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Sano, Kuniaki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Kiyama, Kazuko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Kimura, Hiroshi Osaka Univ, Grad Sch Frontier Biosci, Lab Biol Sci
Kawano, Shinji Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Furuta, Ryohei Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Miyaji, Mary Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Tsutsui, Ken Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
Tsutsui, Kimiko M. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurogen
抄録
DNA topoisomerase II (topo II) changes DNA topology by cleavage/re-ligation cycle(s) and thus contributes to various nuclear DNA transactions. It is largely unknown how the enzyme is controlled in a nuclear context. Several studies have suggested that its C-terminal domain (CTD), which is dispensable for basal relaxation activity, has some regulatory influence. In this work, we examined the impact of nuclear localization on regulation of activity in nuclei. Specifically, human cells were transfected with wild-type and mutant topo II beta tagged with EGFP. Activity attenuation experiments and nuclear localization data reveal that the endogenous activity of topo II beta is correlated with its subnuclear distribution. The enzyme shuttles between an active form in the nucleoplasm and a quiescent form in the nucleolus in a dynamic equilibrium. Mechanistically, the process involves a tethering event with RNA. Isolated RNA inhibits the catalytic activity of topo II beta in vitro through the interaction with a specific 50-residue region of the CTD (termed the CRD). Taken together, these results suggest that both the subnuclear distribution and activity regulation of topo II beta are mediated by the interplay between cellular RNA and the CRD.
発行日
2014-07-17
出版物タイトル
Nucleic Acids Research
42巻
14号
出版者
Oxford University Press
開始ページ
9005
終了ページ
9020
ISSN
0305-1048
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/53130
言語
English
著作権者
© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.
論文のバージョン
publisher
査読
有り
DOI
Web of Science KeyUT