ID | 30743 |
JaLCDOI | |
フルテキストURL | |
著者 |
Sumiyoshi, Hideaki
Oita University
Matsuo, Noritaka
Oita University
Shin, Toshitaka
Oita University
Shirabe, Komei
Oita University
Yoshioka, Hidekatsu
Oita University
|
抄録 | Type III collagen is found in fetal skin and blood vessels. Previously, we characterized the proximal promoter of the human alpha1(III) collagen gene (COL3A1) using the human rhabdomyosarcoma cell line, A204, and NIH3T3 cells (Yoshino et al., Biochim Biophys Acta, 2005). In the present study, we further analyzed this promoter using additional cell lines, namely a human embryonal rhabdomyosarcoma cell line (RD) and bovine vascular smooth muscle cells (vSMCs), both of which show high expression of type III collagen. Using a luciferase assay, electrophoretic mobility shift assays (EMSA), and DNase footprinting assay, 2 types of multifactor complexes were shown to bind to the DNA region in the vicinity of the B element (- 80 to - 58), depending on the cell type. Next, we used cells stably transfected with a GFP-linked type III collagen promoter fragment for analysis of promoter expression. Usually, transfected cells retained the characteristics of the original cells. However, in several clones derived from RD cells, promoter expression as well as cell shape changed to patterns characteristic of the A204 cell line. Nuclear factors expressed by these clones were also characteristic of the A204 line. |
キーワード | type III collagen
promoter
transcription
DNA binding protein
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2006-06
|
巻 | 60巻
|
号 | 3号
|
出版者 | Okayama University Medical School
|
開始ページ | 181
|
終了ページ | 189
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |