ID | 32850 |
JaLCDOI | |
フルテキストURL | |
著者 |
Wu, Yun-Feng
Department of Matrix Medicine, Faculty of Medicine, Oita University
Matsuo, Noritaka
Department of Matrix Medicine, Faculty of Medicine, Oita University
Sumiyoshi, Hideaki
Department of Matrix Medicine, Faculty of Medicine, Oita University
Yoshioka, Hidekatsu
Department of Matrix Medicine, Faculty of Medicine, Oita University
|
抄録 | The purpose of this study was to clarify the mechanism responsible for the transcriptional regulation of the mouse Col5a3 gene in osteoblastic cells. Transient transfection into rat osteosarcoma ROS17/2.8 cells demonstrated that a region from nucleotides 337 to 1 was involved in the transcriptional activity of the Col5a3 gene. An electrophoretic mobility shift assay showed that Sp1/Sp3 and CBF/NF-Y bound to a GC-rich domain (194/186) and a CCAAT box (134/130) in the Col5a3 gene, respectively. Introduction of mutations or deletion into a GC-rich domain, the CCAAT box, or both elements decreased the transcription activity. Overexpression of Sp1 increases the transcription activity and interferes with Sp family binding to the GC-rich domain to decrease promoter activity. Therefore, the transcription of the mouse Col5a3 gene is cooperatively regulated by Sp1 and CBF/NF-Y in osteoblastic cells. |
キーワード | type V collagen
Sp1
CBF/NF-Y
gene regulation
osteoblastic cell
|
Amo Type | Original Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2010-04
|
巻 | 64巻
|
号 | 2号
|
出版者 | Okayama University Medical School
|
開始ページ | 95
|
終了ページ | 108
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
Web of Science KeyUT |