ID | 30730 |
JaLCDOI | |
フルテキストURL | |
著者 |
Arai, Meiji
Jichi Medical School
Kosuge, Kazuko
Jichi Medical School
Kawamoto, Fumihiko
Oita University
Matasuoka, Hiroyuki
Jichi Medical School
|
抄録 | Deficiency of glucose-6-phosphate dehydrogenase (G6PD) causes acute hemolytic anemia triggered by oxidative drugs such as primaquine. It is therefore essential in malaria-endemic areas for malaria patients to be confirmed for their G6PD activity before taking primaquine. The WST-8 method, a newly established screening method for G6PD deficiency, has been demonstrated to be suitable for field conditions, particularly for on-site malaria surveys. Here we report a laboratory evaluation by this method of the reactivity of blood-spotted filters. A time-course experiment was conducted to evaluate the reactivity of blood samples spotted onto 4 types of filter paper, Whatman 31ET Chr (ET), 3MM Chr (3MM), P81, and Advantec No. 2 (AD2). The rank of the relative reaction intensity was ET > 3MM = AD2 > P81. Blood-spotted filters stored at 4 degrees centigrade gradually decreased G6PD reactivity with the passage of storage time, whereas those stored at room temperature rapidly reduced their reactivity. Unexpectedly, saponin supplementation reduced the reactivity of blood-spotted filters. In conclusion, 1) ET is the most suitable filter for the WST-8 method ; 2) blood-spotted filters stored in cold condition can be assayed within 14 days, or those stored at room temperature should be tested within 3 days ; and 3) reaction mixtures should not contain saponin. |
キーワード | glucose-6-phosphate dehydrogenase
G6PD deficiency
blood-spotted filter
WST-8 method
malaria
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2006-04
|
巻 | 60巻
|
号 | 2号
|
出版者 | Okayama University Medical School
|
開始ページ | 127
|
終了ページ | 134
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |