ID | 31068 |
JaLCDOI | |
フルテキストURL | |
著者 |
Terato, Kuniaki
Okayama University
Kawanishi, Koichi
Okayama University
Yamamoto, Shinro
Okayama University
|
抄録 | In urine of diabetics, a significantly great inhibitory activity of glyceraldehyde 3-phosphate dehydrogenase (GAP-Dehyd) was observed compared with that of normal subjects. The 0.2 ml of urine from 41 patients with diabetes mellitus inhibited 0.5 units of GAP-Dehyd by 27.2 +/- 3.0% (mean +/- S.E.M.), while that from 17 normal volunteers inhibited only by 9.0 +/- 1.0% (P less than 0.05). This inhibitory substance was extracted by 90% ethanol from diabetic urine and partially purified by anion exchange chromatography using Dowex-1 (HCOO type). The molecular weight of this substance was confirmed to be 100--300 daltons by an analysis on Biogel P-4 gel filtration chromatography. And analysis by thin layer chromatography using silicagel plate showed that this inhibitor was a ninhydrin reactive substance which has not been reported previously. From the above facts, it was assumed that the inhibitory substance of GAP-Dehyd in urine of diabetics was a new acidic compound of low molecular weight containing an amino residue in the molecule. |
キーワード | glyceraldehyde 3-phosphate dehydrogenase
inhibitor
diabetes mellitus
urine
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1978-10
|
巻 | 32巻
|
号 | 5号
|
出版者 | Okayama University Medical School
|
開始ページ | 337
|
終了ページ | 342
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
NAID |