ID | 31062 |
JaLCDOI | |
フルテキストURL | |
著者 |
Miyazaki, Masahiro
Okayama University
Watanabe, Akiharu
Okayama University
Shiraishi, Masayuki
Okayama University
Hoshika, Teruki
Okayama University
Miyano, Keiko
Okayama University
Sato, Jiro
Okayama University
|
抄録 | The effect of various hormones on cytological and biochemical properties of cultured hepatic cells were investigated in order to obtain long-term survival of the hepatocytes with adult liver functions in primary culture. Insulin supplementation of the culture medium enhanced the attachemnt efficiency of cells in primary culture without affecting either maintenance of morphological characters of epithelial cells or retention of liver-specific functions in cultured cells. A combination of dexamethasone and insulin was apparently effective in stimulating the formation of a monolayer of polygonal cells with granular cytoplasm and in maintenance of liver-specific functions for relatively longer periods. Supplementation with either dexamethasone or hydrocortisone alone enhanced tyrosine aminotransferase activities in cultured cells for at least 4 days postinoculation. These steroid hormones also allowed growth of small epithelial cells with clear cytoplasm and maintenance of increased albumin production for 8 days after inoculation. The roles of these hormones in the primary culture of isolated hepatic cells are discussed in the present paper. |
キーワード | primary culture
adult rat hepatic cells
hormones
albumin
tyrosine aminotransferase
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1978-06
|
巻 | 32巻
|
号 | 2号
|
出版者 | Okayama University Medical School
|
開始ページ | 85
|
終了ページ | 96
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
NAID |