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ID 31084
JaLCDOI
フルテキストURL
fulltext.pdf 1.18 MB
著者
Seki, Shuji Okayama University
Nakamura, Takashi Okayama University
Kataoka, Kazuhiro Okayama University
Oda, Takuzo Okayama University
抄録

Previous investigation showed that mouse ascites sarcoma cells permeabilized with appropriate concentrations of detergents (Triton X-100, Nonidet P-40 and Brij 58) had high replicative DNA synthesis in the presence of the four deoxyribonucleoside triphosphates, ATP, Mg2+ and proper ionic environment. The present study showed the optimum detergent concentration for DNA synthesis coincided closely with the minimum detergent concentration for inducing cell swelling. Phase contrast microscopy and electron microscopy of Triton-permeabilized cells showed the characteristic swollen cytoplasms and nucleus. Autoradiographic study showed that the DNA synthesis in permeable cells was confined to the nucleus. Cell viability and [3H] deoxythymidine uptake were impaired at much lower concentrations of Triton X-100 than the optimum concentration for in vitro DNA synthesis. In Triton-permeabilized cells, the minimum Triton concentration that produced cell swelling also seemed to produce high repliative DNA synthesis, which reflects the in vivo state of DNA synthesis.

キーワード
cell swelling
DNA synthesis
permeable cells
Amo Type
Article
出版物タイトル
Acta Medica Okayama
発行日
1978-04
32巻
1号
出版者
Okayama University Medical School
開始ページ
1
終了ページ
9
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
英語
論文のバージョン
publisher
査読
有り
PubMed ID
NAID