ID | 60716 |
フルテキストURL | |
著者 |
Yoshihara, Kumiko
Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Pathology & Experimental Medicine
Nagaoka, Noriyuki
Okayama University Dental School, Advanced Research Center for Oral and Craniofacial Sciences
Nakamura, Akiko
National Institute for Materials Science
Hara, Toru
National Institute for Materials Science
Hayakawa, Satoshi
Okayama University, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Biomaterials Laboratory
ORCID
Kaken ID
publons
researchmap
Yoshida, Yasuhiro
Hokkaido University, Faculty of Dental Medicine, Department of Biomaterials and Bioengineering
Van Meerbeek, Meerbeek, Bart
KU Leuven (University of Leuven), Department of Oral Health Research, BIOMAT & UZ Leuven (University Hospitals Leuven)
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抄録 | The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in the caries lesions were characterized by transmission electron microscopy (TEM), along with chemical element mapping using energy-dispersive spectroscopy (EDS)-STEM. FIB-SEM 3D reconstructions revealed a severely damaged dentin surface abundantly covered by bacteria. Although the dentin tubules were clogged up to a depth of 100 mu m, bacterial invasion into dentin tubules was not observed. TEM crystal analysis and EDS-STEM revealed the presence of Ca and P, as well as of Mg within the HAp crystals deposited inside the dentin tubules. It was concluded that extensive remineralization with deposition of Mg-HAp crystals had occurred in dentin tubules of caries-arrested dentin. Understanding the natural remineralization process is thought to be helpful for developing clinical biomimetic remineralization protocols.
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発行日 | 2020-03-09
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出版物タイトル |
Scientific Reports
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巻 | 10巻
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号 | 1号
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出版者 | Nature
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開始ページ | 4387
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ISSN | 2045-2322
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2020
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1038/s41598-020-61111-1
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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助成機関名 |
日本学術振興会
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助成番号 | 16K2045508
18K17068
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