ID | 60712 |
フルテキストURL | |
著者 |
Kojima, Keiichi
Faculty of Pharmaceutical Sciences, Okayama University
Ueta, Tetsuya
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Noji, Tomoyasu
Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo
Saito, Keisuke
Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo
Kanehara, Kanae
Faculty of Pharmaceutical Sciences, Okayama University
Yoshizawa, Susumu
Atmosphere and Ocean Research Institute, The University of Tokyo
Ishikita, Hiroshi
Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo
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抄録 | Rubrobacter xylanophilus rhodopsin (RxR) is a phylogenetically distinct and thermally stable seven-transmembrane protein that functions as a light-driven proton (H+) pump with the chromophore retinal. To characterize its vectorial proton transport mechanism, mutational and theoretical investigations were performed for carboxylates in the transmembrane region of RxR and the sequential proton transport steps were revealed as follows: (i) a proton of the retinylidene Schiff base (Lys209) is transferred to the counterion Asp74 upon formation of the blue-shifted M-intermediate in collaboration with Asp205, and simultaneously, a respective proton is released from the proton releasing group (Glu187/Glu197) to the extracellular side, (ii) a proton of Asp85 is transferred to the Schiff base during M-decay, (iii) a proton is taken up from the intracellular side to Asp85 during decay of the red-shifted O-intermediate. This ion transport mechanism of RxR provides valuable information to understand other ion transporters since carboxylates are generally essential for their functions.
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キーワード | Biochemistry
Biophysics
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発行日 | 2020-01-14
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出版物タイトル |
Scientific Reports
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巻 | 10巻
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号 | 1号
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出版者 | Nature
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開始ページ | 282
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ISSN | 2045-2322
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2020
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1038/s41598-019-57122-2
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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助成機関名 |
Japan Agency for Medical Research and Development
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助成番号 | 18ak0101084h0002
18dm0207060h0002
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