ID | 51875 |
フルテキストURL | |
著者 |
Kikuchi, Takeshi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Kubota, Satoshi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Kaken ID
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Asaumi, Koji
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Kawaki, Harumi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Nishida, Takashi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
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Kawata, Kazumi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Mitani, Shigeru
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Tabata, Yasuhiko
Kyoto Univ, Inst Frontier Med Sci, Dept Biomat
Ozaki, Toshifumi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Kaken ID
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Takigawa, Masaharu
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Kaken ID
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抄録 | CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique molecule that promotes the entire endochondral ossification process and regeneration of damaged articular cartilage. Also, CCN2 has been shown to enhance the adhesion and migration of bone marrow stromal cells as well as the growth and differentiation of osteoblasts; hence, its utility in bone regeneration has been suggested. Here, we evaluated the effect of CCN2 on the regeneration of an intractable bone defect in a rat model. First, we prepared two recombinant CCN2s of different origins, and the one showing the stronger effect on osteoblasts in vitro was selected for further evaluation, based on the result of an in vitro bioassay. Next, to obtain a sustained effect, the recombinant CCN2 was incorporated into gelatin hydrogel that enabled the gradual release of the factor. Evaluation in vivo indicated that CCN2 continued to be released at least for up to 14 days after its incorporation. Application of the gelatin hydrogel-CCN2 complex, together with a collagen scaffold to the bone defect prepared in a rat femur resulted in remarkable induction of osteoblastic mineralization markers within 2 weeks. Finally, distinct enhancement of bone regeneration was observed 3 weeks after the application of the complex. These results confirm the utility of CCN2 in the regeneration of intractable bone defects in vivo when the factor is incorporated into gelatin hydrogel.
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発行日 | 2008-06
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出版物タイトル |
Tissue Engineering Part A
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巻 | 14巻
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号 | 6号
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出版者 | Mary Ann Liebert, Inc.
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開始ページ | 1089
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終了ページ | 1098
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ISSN | 1937-3341
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1089/ten.tea.2007.0167
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言語 |
英語
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著作権者 | © Mary Ann Liebert, Inc.
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
PubMed ID | |
Web of Science KeyUT |