ID | 65101 |
フルテキストURL | |
著者 |
Inada, Yasunori
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Takabatake, Kiyofumi
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Tsujigiwa, Hidetsugu
Department of Life Science, Faculty of Science, Okayama University of Science
Nakano, Keisuke
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Shan, Qiusheng
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Piao, Tianyan
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Chang, Anqi
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kawai, Hotaka
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nagatsuka, Hitoshi
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kaken ID
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抄録 | Bone marrow is complex structure containing heterogenetic cells, making it difficult to regenerate using artificial scaffolds. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which is a cylindrical scaffold with a honeycomb arrangement of straight pores, and we demonstrated that TCP with 300 and 500 mu m pore diameters (300TCP and 500TCP) induced bone marrow structure within the pores. In this study, we examined the optimal scaffold structure for bone marrow with homeostatic bone metabolism using honeycomb TCP. 300TCP and 500TCP were transplanted into rat muscle, and bone marrow formation was histologically assessed. Immunohistochemistry for CD45, CD34, Runt-related transcription factor 2 (Runx2), c-kit single staining, Runx2/N-cadherin, and c-kit/Tie-2 double staining was performed. The area of bone marrow structure, which includes CD45(+) round-shaped hematopoietic cells and CD34(+) sinusoidal vessels, was larger in 300TCP than in 500TCP. Additionally, Runx2(+) osteoblasts and c-kit(+) hematopoietic stem cells were observed on the surface of bone tissue formed within TCP. Among Runx2(+) osteoblasts, spindle-shaped N-cadherin(+) cells existed in association with c-kit(+)Tie-2(+) hematopoietic stem cells on the bone tissue formed within TCP, which formed a hematopoietic stem cell niche similar to as in vivo. Therefore, honeycomb TCP with 300 mu m pore diameters may be an artificial scaffold with an optimal geometric structure as a scaffold for bone marrow formation.
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キーワード | honeycomb TCP
bone marrow formation
scaffold
hematopoietic stem cell (HSC)
N-cadherin-positive spindle-shaped osteoblasts (SNO cells)
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発行日 | 2023-02-07
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出版物タイトル |
Materials
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巻 | 16巻
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号 | 4号
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出版者 | MDPI
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開始ページ | 1393
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ISSN | 1996-1944
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2023 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/ma16041393
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Inada, Y.; Takabatake, K.; Tsujigiwa, H.; Nakano, K.; Shan, Q.; Piao, T.; Chang, A.; Kawai, H.; Nagatsuka, H. Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate. Materials 2023, 16, 1393. https://doi.org/10.3390/ ma16041393
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | JP20H03888
JP21K10043
JP21K17089
JP22K10170
JP19K19159
JP20K10178
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