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ID 55567
フルテキストURL
著者
Ogawa, Hirohito Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kajihara, Masahiro Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University
Nao, Naganori Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University
Shigeno, Asako Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University
Fujikura, Daisuke Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University
Hang’ombe, Bernard M. Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia
Mweene, Aaron S. Department of Disease Control, School of Veterinary Medicine, University of Zambia
Mutemwa, Alisheke Provincial Veterinary Office, Department of Veterinary Services, Ministry of Fisheries and Livestock
Squarre, David Department of National Parks and Wildlife, Ministry of Tourism and Arts
Yamada, Masao Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Higashi, Hideaki Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University
Sawa, Hirofumi Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University
Takada, Ayato Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University
抄録
Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name "Bat mastadenovirus H". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.
キーワード
Eidolon helvum
Zambia
adenovirus
bat
発行日
2017-12-04
出版物タイトル
Viruses
9巻
12号
出版者
MDPI
開始ページ
371
ISSN
1999-4915
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン
publisher
PubMed ID
DOI
関連URL
isVersionOf https://doi.org/10.3390/v9120371