ID | 63809 |
フルテキストURL | |
著者 |
Juma, Patrick Otieno
Institute of Plant Science and Resources, Okayama University
Fujitani, Yoshiko
Institute of Plant Science and Resources, Okayama University
Alessa, Ola
Institute of Plant Science and Resources, Okayama University
Oyama, Tokitaka
Graduate School of Science, Kyoto University
Yurimoto, Hiroya
Graduate School of Agriculture, Kyoto University
Sakai, Yasuyoshi
Graduate School of Agriculture, Kyoto University
Tani, Akio
Institute of Plant Science and Resources, Okayama University
ORCID
Kaken ID
publons
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抄録 | Methylobacterium and Methylorubrum species are facultative methylotrophic bacteria that are abundant in the plant phyllosphere. They have two methanol dehydrogenases, MxaF and XoxF, which are dependent on either calcium or lanthanides (Lns), respectively. Lns exist as insoluble minerals in nature, and their solubilization and uptake require a siderophore-like substance (lanthanophore). Methylobacterium species have also been identified as plant growth-promoting bacteria although the actual mechanism has not been well-investigated. This study aimed to reveal the roles of siderophore in Methylobacterium aquaticum strain 22A in Ln uptake, bacterial physiology, and plant growth promotion. The strain 22A genome contains an eight-gene cluster encoding the staphyloferrin B-like (sbn) siderophore. We demonstrate that the sbn siderophore gene cluster is necessary for growth under low iron conditions and was complemented by supplementation with citrate or spent medium of the wild type or other strains of the genera. The siderophore exhibited adaptive features, including tolerance to oxidative and nitrosative stress, biofilm formation, and heavy metal sequestration. The contribution of the siderophore to plant growth was shown by the repressive growth of duckweed treated with siderophore mutant under iron-limited conditions; however, the siderophore was dispensable for strain 22A to colonize the phyllosphere. Importantly, the siderophore mutant could not grow on methanol, but the siderophore could solubilize insoluble Ln oxide, suggesting its critical role in methylotrophy. We also identified TonB-dependent receptors (TBDRs) for the siderophore-iron complex, iron citrate, and Ln, among 12 TBDRs in strain 22A. Analysis of the siderophore synthesis gene clusters and TBDR genes in Methylobacterium genomes revealed the existence of diverse types of siderophores and TBDRs. Methylorubrum species have an exclusive TBDR for Ln uptake that has been identified as LutH. Collectively, the results of this study provide insight into the importance of the sbn siderophore in Ln chelation, bacterial physiology, and the diversity of siderophore and TBDRs in Methylobacterium species.
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キーワード | Methylobacterium species
lanthanide
lanthanophore
siderophore
plant growth promoter
heavy metal sequestration
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発行日 | 2022-07-07
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出版物タイトル |
Frontiers In Microbiology
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巻 | 13巻
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出版者 | Frontiers Media SA
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開始ページ | 921635
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ISSN | 1664-302X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2022 Juma, Fujitani, Alessa, Oyama, Yurimoto, Sakai and Tani.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3389/fmicb.2022.921635
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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