ID | 62364 |
フルテキストURL | |
著者 |
Liang, Yin
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Wang, Mengxue
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Liu, Yun
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Wang, Chen
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Takahashi, Ken
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
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Naruse, Keiji
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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抄録 | Gravity affects the function and maintenance of organs, such as bones, muscles, and the heart. Several studies have used DNA microarrays to identify genes with altered expressions in response to gravity. However, it is technically challenging to combine the results from various microarray datasets because of their different data structures. We hypothesized that it is possible to identify common changes in gene expression from the DNA microarray datasets obtained under various conditions and methods. In this study, we grouped homologous genes to perform a meta-analysis of multiple vascular endothelial cell and skeletal muscle datasets. According to the t-distributed stochastic neighbor embedding (t-SNE) analysis, the changes in the gene expression pattern in vascular endothelial cells formed specific clusters. We also identified candidate genes in endothelial cells that responded to gravity. Further, we exposed human umbilical vein endothelial cells (HUVEC) to simulated microgravity (SMG) using a clinostat and measured the expression levels of the candidate genes. Gene expression analysis using qRT-PCR revealed that the expression level of the prostaglandin (PG) transporter gene SLCO2A1 decreased in response to microgravity, consistent with the meta-analysis of microarray datasets. Furthermore, the direction of gravity affected the expression level of SLCO2A1, buttressing the finding that its expression was affected by gravity. These results suggest that a meta-analysis of DNA microarray datasets may help identify new target genes previously overlooked in individual microarray analyses.
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キーワード | DNA microarrays
meta-analysis
microgravity
human umbilical vein endothelial cells
prostaglandin transporter
clinostat
spaceflight-associated neuro-ocular syndrome
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発行日 | 2021-08-04
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出版物タイトル |
Frontiers In Cell and Developmental Biology
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巻 | 9巻
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出版者 | Frontiers Media SA
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開始ページ | 689662
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ISSN | 2296-634X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2021 Liang, Wang, Liu, Wang, Takahashi and Naruse.
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論文のバージョン | publisher
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DOI | |
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関連URL | isVersionOf https://doi.org/10.3389/fcell.2021.689662
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Liang Y, Wang M, Liu Y, Wang C, Takahashi K and Naruse K (2021) Meta-Analysis-Assisted Detection of Gravity-Sensitive Genes in Human Vascular Endothelial Cells. Front. Cell Dev. Biol. 9:689662. doi: 10.3389/fcell.2021.689662
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | 15H05936
20K21896
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オープンアクセス(出版社) |
OA
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