ID | 46981 |
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Sort Key | 8
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フルテキストURL | |
著者 |
Yamagiwa, Masashi
Department of Bioscience and Biotechnology Okayama University
Sakai, Hiroshi
Department of Bioscience and Biotechnology Okayama University
Kaken ID
publons
researchmap
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抄録 | An active form of Cry4A is a heterodimer of the 20- and 45-kDa fragments that are derived from the 130-kDa Cry4A protoxin. To investigate the function of these two fragments, several deletion mutants were constructed and expressed in E.coli as the GST (glutathione-S-transferase) fusion proteins. The results of the bioassay against Culex pipiens larvae showed that the interaction of two fragments of Cry4A was necessary for the toxicity, and that the C-terminal 67 amino acids of the 20-kDa fragment corresponding to the helices α4 and α5 were involved in determining the insecticidal activity. Surprisingly the lack of helix α5 did not affect the toxicity to C. pipiens, suggesting that the role of helix α5 of Cry4A was different from that postulated in the case of Cry4A toxins.
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出版物タイトル |
Memoirs of the Faculty of Engineering, Okayama University
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発行日 | 2003-03
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巻 | 37巻
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号 | 2号
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出版者 | Faculty of Engineering, Okayama University
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開始ページ | 67
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終了ページ | 72
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ISSN | 0475-0071
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NCID | AA10699856
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資料タイプ |
紀要論文
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OAI-PMH Set |
岡山大学
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言語 |
英語
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論文のバージョン | publisher
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NAID | |
Eprints Journal Name | mfe
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