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ID 47026
フルテキストURL
著者
Yamagiwa, Masashi Department of Bioscience and Biotechnology, Okayama University
Namba, Akitoshi Department of Bioscience and Biotechnology, Okayama University
Akao, Tetsuyuki Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
Mizuki, Eiichi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
Ohba, Michio Graduate School of Agriculture, Kyusyu University
Sakai, Hiroshi Department of Bioscience and Biotechnology, Okayama University
抄録
The crystal proteins produced by Bacillus thuringiensis subsp, israelensis (Bti) and subsp. coreanensis A1519 strain were examined for the cytotoxicity against MOLT-4 and HeLa cells by MTT assay and LDH assay, The A1519 crystal proteins processed by proteinase K exhibited the specific cell-killing activity toward MOLT-4 with little damage to the cell membrane, On the other hand, the Bti crystal proteins processed by proteinase K caused the substantial damage to the cell membrane of both MOLT-4 and HeLa, leading to the cell lysis. The non-digested crystal proteins of both strains exhibited no cytotoxicity, These data suggested that while the Bti crystal proteins caused the colloid-osmotic swelling and cell lysis of MOLT-4 and HeLa, the proteinase K-digested A1519 crystal proteins induced the specific cell death of MOLT-4 through a mechanism other than that of Bti.
発行日
2002-03
出版物タイトル
Memoirs of the Faculty of Engineering, Okayama University
36巻
2号
出版者
Faculty of Engineering, Okayama University
開始ページ
61
終了ページ
66
ISSN
0475-0071
NCID
AA10699856
資料タイプ
紀要論文
言語
English
論文のバージョン
publisher
査読
無し
Eprints Journal Name
mfe