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ID 34234
フルテキストURL
Thumnail figs.pdf 2.84 MB
著者
Morimoto, Riyo Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Uehara, Shunsuke Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Yatsushiro, Shouki Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Juge, Narinobu Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Hua, Zhaolin Departments of Neurology and Physiology, Graduate Programs in Neuroscience and Cell Biology, University of California San Francisco School of Medicine
Senoh, Shigenori Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Echigo, Noriko Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Hayashi, Mitsuko Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Mizoguchi, Toshihide Institute for Oral Science, Matsumoto Dental University
Ninomiya, Tadashi Institute for Oral Science, Matsumoto Dental University
Udagawa, Nobuyuki Department of Biochemistry, Matsumoto Dental University
Omote, Hiroshi Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Yamamoto, Akitsugu Department of Cell Biology, Nagahama Institute of Bioscience and Technology
Edwards, Robert H Departments of Neurology and Physiology, Graduate Programs in Neuroscience and Cell Biology, University of California San Francisco School of Medicine
Moriyama, Yoshinori Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
抄録
Osteoclasts are involved in the catabolism of the bone matrix and eliminate the resulting degradation products through transcytosis, but the molecular mechanism and regulation of transcytosis remain poorly understood. Upon differentiation, osteoclasts express vesicular glutamate transporter 1 (VGLUT1), which is essential for vesicular storage and subsequent exocytosis of glutamate in neurons. VGLUT1 is localized in transcytotic vesicles and accumulates L-glutamate. Osteoclasts secrete L-glutamate and the bone degradation products upon stimulation with KCl or ATP in a Ca2+-dependent manner. KCl- and ATP-dependent secretion of L-glutamate was absent in osteoclasts prepared from VGLUT1-/- knockout mice. Osteoclasts express mGluR8, a class III metabotropic glutamate receptor. Its stimulation by a specific agonist inhibits secretion of L-glutamate and bone degradation products, whereas its suppression by a specific antagonist stimulates bone resorption. Finally, it was found that VGLUT1-/- mice develop osteoporosis. Thus, in bone-resorbing osteoclasts, L-glutamate and bone degradation products are secreted through transcytosis and the released L-glutamate is involved in autoregulation of transcytosis. Glutamate signaling may play an important role in the bone homeostasis.
キーワード
osteoclast
vesicular glutamate transporter
transcytosis
bone resorption
備考
Digital Object Identifer:10.1038/sj.emboj.7601317
Published with permission from the copyright holder. This is the author's copy, as published in EMBO Journal, Sep 2006, Volume 25, Issue 18, Pages 4175-4186.
Publisher URL:http://dx.doi.org/10.1038/sj.emboj.7601317
Direct access to Thomson Web of Science record
Copyright © 2006 European Molecular Biology Organization
発行日
2006-09-20
出版物タイトル
EMBO Journal
25巻
18号
出版者
European Molecular Biology Organization
開始ページ
4175
終了ページ
4186
ISSN
0261-4189
NCID
AA10627582
資料タイプ
学術雑誌論文
言語
English
著作権者
European Molecular Biology Organization
論文のバージョン
author
査読
有り
DOI
Submission Path
biochemistry/7