ID | 57810 |
フルテキストURL | |
著者 |
Matsuura, Yuki
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Ueda, Masashi
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
ORCID
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Higaki, Yusuke
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Kaken ID
Watanabe, Keiko
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Habara, Shogo
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Kamino, Shinichiro
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
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Saji, Hideo
Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University
Enomoto, Shuichi
Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
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抄録 | INTRODUCTION:
Nicotinic acetylcholine receptors (nAChRs) are of great interest because they are implicated in higher brain functions. Nuclear medical imaging is one of the useful techniques for noninvasive evaluation of physiological and pathological function in living subjects. Recent progress in nuclear medical imaging modalities enables the clear visualization of the organs of small rodents. Thus, translational research using nuclear medical imaging in transgenic mice has become possible and helps to elucidate human disease pathology. However, imaging of α4β2 nAChRs in the mouse brain has not yet been performed. The purpose of this study was to assess the feasibility of single-photon emission computed tomography (SPECT) with 5-[(123)I]iodo-3-[2(S)-azetidinylmethoxy]pyridine ([(123)I]5IA) for evaluating α4β2 nAChR availability in the mouse brain. METHODS: A 60-min dynamic SPECT imaging session of α4β2 nAChRs in the mouse brain was performed. The regional distribution of radioactivity in the SPECT images was compared to the density of α4β2 nAChRs measured in an identical mouse. Alteration of nAChR density in the brains of Tg2576 mice was also evaluated. RESULTS: The mouse brain was clearly visualized by [(123)I]5IA-SPECT and probe accumulation was significantly inhibited by pretreatment with (-)-nicotine. The regional distribution of radioactivity in SPECT images showed a significant positive correlation with α4β2 nAChR density measured in an identical mouse brain. Moreover, [(123)I]5IA-SPECT was able to detect the up-regulation of α4β2 nAChRs in the brains of Tg2576 transgenic mice. CONCLUSIONS: [(123)I]5IA-SPECT imaging would be a promising tool for evaluating α4β2 nAChR availability in the mouse brain and may be useful in translational research focused on nAChR-related diseases. |
キーワード | 5-[(123)I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([(123)I]5IA)
Alzheimer's disease
Mouse
Nicotinic acetylcholine receptor (nAChR)
Single-photon emission computed tomography (SPECT)
Tg2576
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発行日 | 2016-06
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出版物タイトル |
Nuclear Medicine and Biology
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巻 | 43巻
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号 | 6号
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出版者 | Elsevier Science
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開始ページ | 372
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終了ページ | 378
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ISSN | 0969-8051
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NCID | AA10864894
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | author
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PubMed ID | |
DOI | |
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関連URL | isVersionOf https://doi.org/10.1016/j.nucmedbio.2016.02.007
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Citation | Yuki Matsuura, Masashi Ueda, Yusuke Higaki, Keiko Watanabe, Shogo Habara, Shinichiro Kamino, Hideo Saji, Shuichi Enomoto, Noninvasive evaluation of nicotinic acetylcholine receptor availability in mouse brain using single-photon emission computed tomography with [123I]5IA, Nuclear Medicine and Biology, Volume 43, Issue 6, 2016, Pages 372-378, ISSN 0969-8051, https://doi.org/10.1016/j.nucmedbio.2016.02.007.
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助成機関名 |
日本学術振興会
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助成番号 | 26670562
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