ID | 56675 |
フルテキストURL | |
著者 |
Mullick, Satarupa
National Institute of Cholera and Enteric Diseases
Mandal, Paulami
National Institute of Cholera and Enteric Diseases
Mukti Kant Nayak
National Institute of Cholera and Enteric Diseases
Ghosh, Souvik
Department of Hygiene, Sapporo Medical University School of Medicine
De, Papiya
National Institute of Cholera and Enteric Diseases
Rajendran, K.
National Institute of Cholera and Enteric Diseases
Bhattacharya, Mihir K.
National Institute of Cholera and Enteric Diseases
Mitra, Utpala
National Institute of Cholera and Enteric Diseases
Ramamurthy, Thandavarayan
National Institute of Cholera and Enteric Diseases
Kobayashi, Nobumichi
Department of Hygiene, Sapporo Medical University School of Medicine
Chawla-Sarkar, Mamta
National Institute of Cholera and Enteric Diseases
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抄録 | INTRODUCTION:
India accounts for an estimated 457,000-884,000 hospitalizations and 2 million outpatient visits for diarrhea. In spite of the huge burden of rotavirus (RV) disease, RV vaccines have not been introduced in national immunization programme of India. Therefore, continuous surveillance for prevalence and monitoring of the circulating genotypes is needed to assess the disease burden prior to introduction of vaccines in this region.
METHODS:
During January 2011 through December 2013, 830 and 1000 stool samples were collected from hospitalized and out-patient department (OPD) patients, respectively, in two hospitals in Kolkata, Eastern India. After primary screening, the G-P typing was done by multiplex semi-nested PCR using type specific primers followed by sequencing. Phylogenetic analysis for the VP7 gene of 25 representative strains was done.
RESULTS:
Among hospitalized and OPD patients, 53.4% and 47.5% cases were positive for rotaviruses, respectively. Unlike previous studies where G1 was predominant, in hospitalized cases G9 rotavirus strains were most prevalent (40%), followed by G2 (39.6%) whereas G1 and G12 occurred at 16.4% and 5.6% frequency. In OPD cases, the most prevalent strain was G2 (40.3%), followed by G1, G9 and G12 at 25.5%, 22.8%, 9.3%, respectively. Phylogenetically the G1, G2 and G9 strains from Kolkata did not cluster with corresponding genotypes of Rotarix, RotaTeq and Rotavac (116E) vaccine strains.
CONCLUSION:
The study highlights the high prevalence of RV in children with gastroenteritis in Kolkata. The circulating genotypes have changed over the time with predominance of G9 and G2 strains during 2011-2013. The current G2, G9 and G1 Kolkata strains shared low amino acid homologies with current vaccine strains. Although there is substantial evidence for cross protection of vaccines against a variety of strains, still the strain variation should be monitored post vaccine introduction to determine if it has any impact on vaccine effectiveness.
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キーワード | Diarrhea
Rotavirus
India
Kolkata
G9 strains
G2 strains
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発行日 | 2014-08-11
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出版物タイトル |
Vaccine
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巻 | 32巻
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号 | supplment 1号
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出版者 | Elsevier Science
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開始ページ | A20
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終了ページ | A28
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ISSN | 0264410X
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NCID | AA10491877
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1016/j.vaccine.2014.03.018
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プロジェクト |
インド感染症共同研究
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