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ID 46923
フルテキストURL
著者
Kawakami, Keisuke Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University
Umena, Yasufumi Department of Chemistry, Graduate School of Science, Osaka City University
Iwai, Masako Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
Kawabata, Yousuke Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University
Ikeuchi, Masahiko Department of Life Sciences (Biology), Graduate School of Arts and Science, The University of Tokyo
Kamiya, Nobuo Department of Chemistry, Graduate School of Science, Osaka City University
Shen, Jian-Ren Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University
抄録
PsbM and PsbI are two low molecular weight subunits of photosystem II (PSII), with PsbM being located in the center, and PsbI in the periphery, of the PSII dimer. In order to study the functions of these two subunits from a structural point of view, we crystallized and analyzed the crystal structure of PSII dimers from two mutants lacking either PsbM or PsbI. Our results confirmed the location of these two subunits in the current crystal structure, as well as their absence in the respective mutants. The relative contents of PSII dimers were found to be decreased in both mutants, with a concomitant increase in the amount of PSII monomers, suggesting a destabilization of PSII dimers in both of the mutants. On the other hand, the accumulation level of the overall PSII complexes in the two mutants was similar to that in the wild-type strain. Treatment of purified PSII dimers with lauryldimethylamine N-oxide at an elevated temperature preferentially disintegrated the dimers from the PsbM deletion mutant into monomers and CP43-less monomers, whereas no significant degradation of the dimers was observed from the PsbI deletion mutant. These results indicate that although both PsbM and PsbI are required for the efficient formation and stability of PSII dimers in vivo, they have different roles, namely, PsbM is required directly for the formation of dimers and its absence led to the instability of the dimers accumulated. On the other hand, PsbI is required in the assembly process of PSII dimers in vivo; once the dimers are formed, PsbI was no longer required for its stability.
キーワード
Photosystem II
Mutant
Crystal structure
PsbM
PsbI
Oxygen evolution
発行日
2011-03
出版物タイトル
Biochimica et Biophysica Acta (BBA) - Bioenergetics
1807巻
3号
出版者
Elsevier Science BV.
開始ページ
319
終了ページ
325
ISSN
0005-2728
NCID
AA00564646
資料タイプ
学術雑誌論文
プロジェクト
エネルギー環境新素材拠点
言語
English
著作権者
© 2010 Elsevier B.V. All rights reserved.
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