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ID 49557
フルテキストURL
著者
Candan, Gerile Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Michiue, Hiroyuki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Ishikawa, Sanae Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Fujimura, Atsushi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Hayashi, Keiichiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Uneda, Atsuhito Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Mori, Akiko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Ohmori, Iori Ohmori
Nishiki, Tei-ichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Matsui, Hideki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
Tomizawa, Kazuhito Kumamoto Univ, Fac Life Sci, Dept Mol Physiol
抄録
Topical therapy is the most favored form of treatment for whitening against hyperpigmentation and sunburn because it lends itself to self-administration, patient compliance, and absence of systemic adverse effects. However, transdermal delivery of hydrophilic chemicals is difficult. The main purpose of this study is to develop a delivering system of hydrophilic drugs and proteins across the skin. Hydroquinone (HQ), a well-known tyrosinase inhibitor and antimelanogenesis compound, and enhanced green fluorescent protein (EGFP) were fused with eleven poly-arginine (11R). Both HQ-11R and EGFP-11R were efficiently delivered in B16 cells, a mouse melanoma cell line. HQ-11R was as effective as HQ alone at inhibiting melanin synthesis in B16 cells. EGFP-11R was efficiently delivered into cells of the epidermis with 4-(1-pyrenyl)-butyric acid (PB), a counteranion bearing an aromatic hydrophobic moiety, in vivo, but EGFP alone or EGFP-11R without PB was not. Finally, topical application of HQ-11R with PB significantly inhibited UV irradiation-induced pigmentation in guinea pigs compared with HQ alone. These results suggest that topical therapy using poly-arginine in combination with PB is useful for the delivery of hydrophilic drugs and proteins by the transdermal route.
キーワード
Transdermal delivery
Protein transduction
Poly-arginine
Tat
Hydroquinone
Tyrosinase inhibitor
発行日
2012-09
出版物タイトル
Biomaterials
33巻
27号
出版者
Elsevier Ltd.
開始ページ
6468
終了ページ
6475
ISSN
0142-9612
NCID
AA11522637
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1016/j.biomaterials.2012.04.056
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/49124
言語
English
OAI-PMH Set
岡山大学
著作権者
(c) 2012 Elsevier Ltd. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT