このエントリーをはてなブックマークに追加
ID 52119
フルテキストURL
著者
Muraoka, Takayuki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Soh, Junichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Toyooka, Shinichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac SurgPathol
Aoe, Keisuke Yamaguchi Ube Med Ctr, Natl Hosp Org, Dept Med Oncol & Clin Res
Fujimoto, Nobukazu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Canc & Thorac Surg
Hashida, Shinsuke Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Maki, Yuho Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Tanaka, Norimitsu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Shien, Kazuhiko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Furukawa, Masashi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Yamamoto, Hiromasa Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Asano, Hiroaki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Tsukuda, Kazunori Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Kishimoto, Takumi Okayama Rosai Hosp, Dept Resp Med
Otsuki, Takemi Kawasaki Med Univ, Dept Hyg
Miyoshi, Shinichiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
抄録
Objectives: Malignant pleural mesothelioma (MPM) is an aggressive tumor with a poor prognosis. microRNA-34b/c (miR-34b/c), which plays an important role in the pathogenesis of MPM, is frequently downregulated by DNA methylation in approximately 90% of MPM cases. In this study, we estimated the degree of miR-34b/c methylation in serum-circulating DNA using a digital methylation specific PCR assay (MSP). Materials and methods: A real-time MSP assay was performed using the SYBR Green method. The melting temperature (Tm) of each PCR product was examined using a melting curve analysis. For a digital MSP assay, 40 wells were analyzed per sample. A total of 110 serum samples from 48 MPM cases, 21 benign asbestos pleurisy (BAP) cases, and 41 healthy volunteers (HVs) were examined. Results: Positive range of Tm value for miR-34b/c methylation was defined as 77.71-78.79 degrees C which was the mean 3 standard deviations of 40 wells of a positive control. The number of miR-34b/c methylated wells was counted per sample according to this criterion. The number of miR-34b/c methylated wells in MPM cases was significantly higher than that in BAP cases (P = 0.03) or HVs (P < 0.001). Advanced MPM cases tended to have higher number of miR-34b/c methylated wells than early MPM cases. Receiver-operating characteristic (ROC) curve analysis revealed that three number of miR-34b/c methylated wells per sample was the best cut-off of positivity of MPM with a 67% of sensitivity and a 77% specificity for prediction. The area under the ROC curve was 0.77. Conclusions: Our digital MSP assay can quantify miR-34b/c methylation in serum-circulating DNA. The degree of miR-34b/c methylation in serum-circulating DNA is associated with MPM, suggesting that this approach might be useful for the establishment of a new detection system for MPM.
キーワード
Digital PCR
Malignant pleural mesothelioma
microRNA
miR-34b/c
Methylation
Circulating DNA
発行日
2013-12
出版物タイトル
Lung Cancer
82巻
3号
出版者
Elsevier Ireland Ltd.
開始ページ
485
終了ページ
490
ISSN
0169-5002
NCID
AA10785743
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1016/j.lungcan.2013.09.017
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52509
言語
English
OAI-PMH Set
岡山大学
著作権者
(C) 2013 Elsevier Ireland Ltd. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT